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The power of mumps virus: Matrix protein activates apoptotic pathways in human colorectal cell lines

Solmaz Morovati1, Ali Mohammadi2, Ramin Masoudi1

  • 1Department of Pathobiology, Division of Biotechnology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

Plos One
|December 13, 2023
PubMed

Insights

The mumps virus matrix (M) protein shows potential as a novel colorectal cancer (CRC) therapy. This protein induces apoptosis in CRC cells, offering a new targeted treatment strategy for this widespread disease.

Area of Science:

  • Oncology
  • Virology
  • Molecular Biology

Background:

  • Colorectal cancer (CRC) is a growing global health concern.
  • Novel therapeutic strategies are crucial for effective CRC control.
  • Targeting viral proteins presents a promising avenue for cancer treatment.

Purpose of the Study:

  • To investigate the anticancer potential of the mumps virus matrix (M) protein in colorectal cancer cells.
  • To explore the M protein's role in inducing apoptosis and its interaction with apoptosis-related pathways.

Main Methods:

  • Transfection of SW480 CRC cell lines with the mumps virus M gene.
  • Assessment of cell death, caspase activity, and apoptosis gene expression.
  • Bioinformatics analysis of the M protein for similarities with Bcl-2 family members.

Main Results:

  • Mumps virus M protein treatment induced significant apoptosis in SW480 cells.
  • Upregulation of pro-apoptotic protein expression and activity was observed.
  • Bioinformatics analysis suggested a BH3 motif in the M protein may interact with cellular Bax.

Conclusions:

  • The mumps virus M protein effectively induces apoptosis in colorectal cancer cells.
  • The M protein's BH3 motif is proposed to mediate apoptosis via Bax interaction.
  • Mumps virus M protein represents a potential targeted therapy for colorectal cancer.

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