Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

When photocatalysts learn to store electrons.

Nature chemistry·2026
Same author

Chirality-selective optical transport of nanoparticles in the evanescent field of a nanofibre.

Nature communications·2026
Same author

Specifying the Origin of Chiral Sensitivity through Conformal Nanogap Engineering in a Single Helicoid Gold Nanoparticle.

ACS nano·2026
Same author

Low-Salt Electrochemical Synthesis of H<sub>2</sub>O<sub>2</sub> Enabled by CO<sub>2</sub>‑Mediated Radical Chemistry.

ACS omega·2026
Same author

Synergistic combinations of antimicrobial peptides and conventional antibiotics: A strategy to delay resistance emergence in World Health Organization priority bacteria.

Pharmacological reviews·2025
Same author

Mechanistic Validation of Lifetime Prediction for Chlorine Evolution Electrodes Using Accelerated Lifetime Tests.

ACS applied materials & interfaces·2025

Related Experiment Video

Updated: Jul 13, 2026

Synthesis of Immunotargeted Magneto-plasmonic Nanoclusters
09:43

Synthesis of Immunotargeted Magneto-plasmonic Nanoclusters

Published on: August 22, 2014

15.3K

Selective Targeting of Nanobody-Modified Gold Nanoparticles to Distinct Cell Types.

Yoon Ho Lee1, Himadri Medhi2, Xin Liu2

  • 1Department of Materials Science and Engineering, Seoul National University, Seoul 08826, Republic of Korea.

ACS Applied Materials & Interfaces
|December 13, 2023
PubMed
Summary

Nanobody-modified gold nanoparticles show cell-targeting potential. Intraperitoneal injection, unlike intravenous, directs these nanoparticles to lymph nodes for potential imaging applications.

Keywords:
PET imagingclick reactiongold nanoparticlesnanobodyradioisotope labeling

More Related Videos

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.2K
Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods
10:46

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods

Published on: May 2, 2016

6.9K

Related Experiment Videos

Last Updated: Jul 13, 2026

Synthesis of Immunotargeted Magneto-plasmonic Nanoclusters
09:43

Synthesis of Immunotargeted Magneto-plasmonic Nanoclusters

Published on: August 22, 2014

15.3K
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.2K
Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods
10:46

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods

Published on: May 2, 2016

6.9K

Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Immunology

Background:

  • Nanoparticles offer versatile platforms for targeted delivery.
  • Nanobodies provide high specificity for cellular targets.
  • Gold nanoparticles are suitable for biomedical applications due to their properties.

Purpose of the Study:

  • To develop and characterize nanobody-conjugated gold nanoparticles for targeted delivery.
  • To evaluate the in vitro and in vivo targeting capabilities of these constructs.
  • To assess the biodistribution of radiolabeled nanobody-gold nanoparticles based on administration route.

Main Methods:

  • Click chemistry conjugation of nanobodies to gold nanoparticles (50 nm octahedral and 180 nm chiral).
  • In vitro targeting assays using Class II MHC-specific nanobodies and fluorometric/microscopy detection.
  • In vivo biodistribution studies using positron emission tomography (PET) isotopes (89Zr, 64Cu) after intravenous and intraperitoneal administration.

Main Results:

  • Nanobody conjugation was successful, retaining antigen-binding capacity.
  • In vitro studies demonstrated selective targeting of Class II MHC-positive cells.
  • Intraperitoneal injection of radiolabeled nanobody-gold nanoparticles resulted in significant accumulation in parathymic lymph nodes, unlike intravenous injection which showed liver/spleen accumulation.

Conclusions:

  • Nanobody-modified gold nanoparticles can be selectively targeted.
  • Administration route critically influences nanoparticle biodistribution.
  • Intraperitoneal delivery of nanobody-gold nanoparticles shows promise for lymph node imaging.