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Coumarin-Conjugated Macromolecular Probe for Sequential Stimuli-Mediated Activation
Yu Jiang1, Runqi Li1, Fei Ren1
1School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, China.
Bioconjugate Chemistry
|December 13, 2023
Summary
Researchers developed RF16_Halo, a novel fluorescent material that responds to hydrogen peroxide and pH changes. This dual-stimuli probe enables sequential logic circuits and precise tracking of intracellular proteins.
Area of Science:
- Bioconjugation Chemistry
- Fluorescent Probes
- Macromolecular Materials
Background:
- Protein bioconjugation is vital for developing protein-based assays.
- Fluorescent materials offer sensitive detection methods.
Purpose of the Study:
- To create a dual-stimuli-responsive fluorescent material (RF16_Halo) for biochemical assays.
- To demonstrate its application in sequential logic circuits and intracellular protein tracking.
Main Methods:
- Conjugation of a coumarin derivative (RF16) to HaloTag protein.
- Installation of a H2O2-sensitive boron cage and a pH-labile moiety.
- Evaluation of sequential fluorescence response to H2O2 and pH.
Main Results:
- RF16_Halo exhibits a linear fluorescence increase with H2O2 and a pH-dependent decrease after cleavage.
- The material functions as a sequential logic circuit with an "off-on-off" fluorescence mode.
- RF16 successfully labeled and tracked intracellular IRE1_Halo protein.
Conclusions:
- A novel structural design strategy for dual-responsive fluorescent probes was established.
- RF16_Halo serves as a versatile tool for information encryption and cellular protein tracking.
