Effects of nitrogen supply on hydrogen-oxidizing bacterial enrichment to produce microbial protein: Comparing...

To investigate the effects of nitrogen source supply on microbial protein (MP) production by hydrogen-oxidizing bacteria (HOB) under continuous feed gas provision, a sequencing batch culture comparison (N₂ fixation versus ammonium assimilation) was performed. The results confirmed that even under basic cultivation conditions, N₂-fixing HOB (NF-HOB) communities showed higher levels of CO₂ and N₂ fixation (190.45 mg/L Δ COD_t and 11.75 mg/L Δ TN_biomass) than previously known, with the highest biomass yield being 0.153 g CDW/g COD-H₂. Rich ammonium stimulated MP synthesis and the biomass accumulation of communities (increased by 7.4 ～ 14.3 times), presumably through the enhancement of H₂ and CO₂ absorption. The micro mechanism may involve encouraging the enrichment of species like Xanthobacter and Acinetobacter then raising the abundance of nitrogenase and glutamate synthase to facilitate the nitrogen assimilation. This would provide NF-HOB with ideas for optimizing their MP synthesis activity.