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Oligosaccharide Assembly

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

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Updated: Jul 8, 2025

A Quantitative Glycomics and Proteomics Combined Purification Strategy

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

Selenium-based metabolic oligosaccharide engineering strategy for quantitative glycan detection.

Xiao Tian¹, Lingna Zheng², Changjiang Wang¹

¹State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing, China.

Nature Communications

|December 13, 2023

Summary

Selenium-based metabolic oligosaccharide engineering (SeMOE) enables precise glycan detection and quantification. This new strategy simplifies glycan analysis for broader biological exploration.

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Area of Science:

Biochemistry
Chemical Biology
Analytical Chemistry

Background:

Metabolic oligosaccharide engineering (MOE) is a chemical method for studying glycans.
Current MOE methods face challenges in glycan quantification due to probe accessibility and background signals.

Purpose of the Study:

To develop a novel selenium-based metabolic oligosaccharide engineering (SeMOE) strategy.
To enable mass spectrometric imaging of the glycome with enhanced quantification.

Main Methods:

Integration of elemental analysis with MOE using selenium-containing probes.
Development of new-to-nature SeMOE probes for glycan detection.

Main Results:

SeMOE enables sensitive detection, quantitative measurement, and visualization of glycans.
The strategy is effective across diverse biological contexts.
Bifunctional SeMOE probes allow for multimodality signal readouts.

Conclusions:

SeMOE offers a simplified and convenient approach to glycan analysis.
This method advances the exploration of the glyco-world.