Protoplast formation and the visualization of nuclei in the eukaryotic microbe, Achlya

Protoplasts have been released form 21 h germlings of the aquatic fungus, Achlya. The commercial enzyme preparation Driselase was utilized to digest the hyphal walls. Protoplast release began 20 min after incubation at 25 degrees C, and was completed by 2 hr after enzyme addition. The fluorescent dye mithramycin was utilized to observe nuclei both in tract hyphae and in isolated protoplasts.