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Epidemiological cut-off values for non-O1/ non-O139 Vibrio cholerae disc diffusion data generated by standardised

Peter Smith1, Laëtitia Le Devendec2, Eric Jouy2

  • 1School of Natural Science, University of Galway, Galway H91 TK33, Ireland.

Diseases of Aquatic Organisms
|December 14, 2023
PubMed
Summary

This study establishes crucial epidemiological cut-off values for Vibrio cholerae antibiotic susceptibility testing. These findings aid in accurately interpreting antimicrobial resistance in non-O1/non-O139 V. cholerae isolates.

Keywords:
Antimicrobial susceptibilityDisc diffusionEpidemiological cut-off valuesNormalized resistance interpretationVibrio cholerae

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Area of Science:

  • Microbiology
  • Antimicrobial Resistance
  • Clinical Laboratory Science

Background:

  • Non-O1/non-O139 Vibrio cholerae strains can cause disease, necessitating standardized antimicrobial susceptibility testing.
  • Establishing epidemiological cut-off values is essential for distinguishing resistant from susceptible bacterial populations.
  • Current data for non-O1/non-O139 V. cholerae susceptibility testing lacks standardized interpretation guidelines.

Purpose of the Study:

  • To generate data for setting epidemiological cut-off values for disc-diffusion zone diameters against non-O1/non-O139 V. cholerae.
  • To standardize antimicrobial susceptibility testing for a significant group of V. cholerae isolates.
  • To provide data for international bodies like CLSI and EUCAST.

Main Methods:

  • Antimicrobial susceptibility testing of 147 European non-O1/non-O139 V. cholerae isolates against 19 antibiotics using a standardized disc diffusion method.
  • Incubation of Mueller Hinton agar plates at 35°C for consistent results.
  • Statistical analysis using the normalized resistance interpretation method to calculate epidemiological cut-off values from zone size data.

Main Results:

  • Epidemiological cut-off values were determined for 19 antibiotics, including amoxicillin/clavulanate, amikacin, ampicillin, cefepime, cefotaxime, ceftazidime, chloramphenicol, ciprofloxacin, erythromycin, florfenicol, gentamicin, imipenem, meropenem, nalidixic acid, norfloxacin, streptomycin, tetracycline, trimethoprim, and trimethoprim/sulfamethoxazole.
  • Data aggregation from four laboratories was used, with adjustments for imprecision in two agents.
  • Calculated cut-off values ranged from ≥13 mm (streptomycin) to ≥31 mm (cefotaxime, ciprofloxacin).

Conclusions:

  • The generated zone size data provides a foundation for establishing international consensus epidemiological cut-off values for non-O1/non-O139 V. cholerae.
  • These values will aid in the accurate interpretation of antimicrobial susceptibility testing results, crucial for effective treatment and surveillance.
  • The study supports the standardization of antimicrobial resistance detection in V. cholerae.