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Epidemiological cut-off values for non-O1/ non-O139 Vibrio cholerae disc diffusion data generated by standardised
Peter Smith1, Laëtitia Le Devendec2, Eric Jouy2
1School of Natural Science, University of Galway, Galway H91 TK33, Ireland.
Diseases of Aquatic Organisms
|December 14, 2023
Summary
This study establishes crucial epidemiological cut-off values for Vibrio cholerae antibiotic susceptibility testing. These findings aid in accurately interpreting antimicrobial resistance in non-O1/non-O139 V. cholerae isolates.
Area of Science:
- Microbiology
- Antimicrobial Resistance
- Clinical Laboratory Science
Background:
- Non-O1/non-O139 Vibrio cholerae strains can cause disease, necessitating standardized antimicrobial susceptibility testing.
- Establishing epidemiological cut-off values is essential for distinguishing resistant from susceptible bacterial populations.
- Current data for non-O1/non-O139 V. cholerae susceptibility testing lacks standardized interpretation guidelines.
Purpose of the Study:
- To generate data for setting epidemiological cut-off values for disc-diffusion zone diameters against non-O1/non-O139 V. cholerae.
- To standardize antimicrobial susceptibility testing for a significant group of V. cholerae isolates.
- To provide data for international bodies like CLSI and EUCAST.
Main Methods:
- Antimicrobial susceptibility testing of 147 European non-O1/non-O139 V. cholerae isolates against 19 antibiotics using a standardized disc diffusion method.
- Incubation of Mueller Hinton agar plates at 35°C for consistent results.
- Statistical analysis using the normalized resistance interpretation method to calculate epidemiological cut-off values from zone size data.
Main Results:
- Epidemiological cut-off values were determined for 19 antibiotics, including amoxicillin/clavulanate, amikacin, ampicillin, cefepime, cefotaxime, ceftazidime, chloramphenicol, ciprofloxacin, erythromycin, florfenicol, gentamicin, imipenem, meropenem, nalidixic acid, norfloxacin, streptomycin, tetracycline, trimethoprim, and trimethoprim/sulfamethoxazole.
- Data aggregation from four laboratories was used, with adjustments for imprecision in two agents.
- Calculated cut-off values ranged from ≥13 mm (streptomycin) to ≥31 mm (cefotaxime, ciprofloxacin).
Conclusions:
- The generated zone size data provides a foundation for establishing international consensus epidemiological cut-off values for non-O1/non-O139 V. cholerae.
- These values will aid in the accurate interpretation of antimicrobial susceptibility testing results, crucial for effective treatment and surveillance.
- The study supports the standardization of antimicrobial resistance detection in V. cholerae.
