Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Rab Cascades01:25

Rab Cascades

2.7K
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
2.7K
Telomeres and Telomerase02:41

Telomeres and Telomerase

23.4K
In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded...
23.4K
The Replisome03:01

The Replisome

33.5K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
33.5K
Tail-anchoring of Proteins in the ER Membrane01:45

Tail-anchoring of Proteins in the ER Membrane

3.1K
Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...
3.1K
Yeast Signaling01:28

Yeast Signaling

14.6K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
14.6K
Regulated mRNA Transport02:22

Regulated mRNA Transport

6.3K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RNase P/MRP subunits chaperone telomerase holoenzyme assembly in fission yeast.

EMBO reports·2026
Same author

Cryo-electron microscopy structure of the budding yeast telomerase holoenzyme.

Science (New York, N.Y.)·2026
Same author

A mechanism for telomere-specific telomere length regulation.

Nucleic acids research·2026
Same author

Telomerase RNA Shapes the Evolutionary Diversity of Telomerase Ribonucleoproteins (RNPs).

Cold Spring Harbor perspectives in biology·2025
Same author

Picobirnavirus: how do you find where it's hiding?

Critical reviews in microbiology·2025
Same author

The single-stranded DNA-binding factor SUB1/PC4 alleviates replication stress at telomeres and is a vulnerability of ALT cancer cells.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same journal

GABAergic neurons in medial prefrontal cortex and ventral hippocampus encode information about reward history in male mice.

Cell reports·2026
Same journal

Dynamicity of Topoisomerase 1 binding at super-enhancers regulates TLR-responsive gene expression program in macrophages.

Cell reports·2026
Same journal

Cadherin regulation of endoplasmic reticulum-plasma membrane contact sites.

Cell reports·2026
Same journal

Type I unconventional protein secretion of the SARS-CoV-2 nucleocapsid protein promotes inflammatory cytokine release.

Cell reports·2026
Same journal

Fibroblast MrgprX2/B2 signaling drives hypertrophic scar fibrosis.

Cell reports·2026
Same journal

Disrupted mitochondrial dynamics activate RNA-sensing innate immunity through mitochondrial RNA release.

Cell reports·2026
See all related articles

Related Experiment Video

Updated: Jul 8, 2025

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

13.6K

Ratcheted transport and sequential assembly of the yeast telomerase RNP.

Hannah Neumann1, Louise Bartle2, Erin Bonnell1

  • 1Department of Microbiology and Infectious Diseases, Faculty of Medicine and Health Sciences, Université de Sherbrooke, 3201 Rue Jean Mignault, Sherbrooke, QC J1E 4K8, Canada.

Cell Reports
|December 14, 2023
PubMed
Summary
This summary is machine-generated.

Telomerase ribonucleoprotein (RNP) maturation involves a unique nuclear-cytoplasmic journey. This process ensures the assembly of active telomerase through RNA processing and protein exchange, essential for replenishing telomeric DNA.

Keywords:
CP: Molecular biologyRNP assemblyRNP transporttelomerasetelomere replication

More Related Videos

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
07:18

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Published on: May 15, 2018

10.8K
Author Spotlight: Advancing Techniques and Discoveries in Protein Synthesis and Assembly Through Innovative Mitochondrial Research
09:53

Author Spotlight: Advancing Techniques and Discoveries in Protein Synthesis and Assembly Through Innovative Mitochondrial Research

Published on: June 7, 2024

989

Related Experiment Videos

Last Updated: Jul 8, 2025

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

13.6K
Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
07:18

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Published on: May 15, 2018

10.8K
Author Spotlight: Advancing Techniques and Discoveries in Protein Synthesis and Assembly Through Innovative Mitochondrial Research
09:53

Author Spotlight: Advancing Techniques and Discoveries in Protein Synthesis and Assembly Through Innovative Mitochondrial Research

Published on: June 7, 2024

989

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Telomerase ribonucleoprotein particles (RNPs) are crucial for maintaining telomeric DNA.
  • Telomerase RNP assembly is a complex, multi-step process occurring across subcellular compartments.
  • The precise mechanisms governing telomerase RNP maturation remain incompletely understood.

Purpose of the Study:

  • To elucidate the intricate steps involved in telomerase RNP maturation.
  • To investigate the role of nucleo-cytoplasmic transport in telomerase assembly.
  • To understand the functional implications of RNA processing and protein exchange during maturation.

Main Methods:

  • Co-transcriptional analysis of telomerase components.
  • Investigation of nuclear export and import mechanisms.
  • Biochemical assays to assess RNP activity and composition.

Main Results:

  • Telomerase RNP biogenesis initiates with co-transcriptional association of components.
  • Nuclear export is dependent on specific RNA features and protein interactions.
  • Cytoplasmic processing involves RNA trimming, protein exchange, and catalytic subunit incorporation.
  • Reimport into the nucleus completes the maturation and activation process.

Conclusions:

  • Telomerase maturation follows a unidirectional, ratchet-type pathway involving a single nucleo-cytoplasmic cycle.
  • Irreversible RNA processing events are critical for driving telomerase assembly.
  • This pathway ensures the formation of a functional telomerase RNP for telomere maintenance.