Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy01:30

Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy

335
Various diagnostic tests are employed in the diagnostic process for Inflammatory Bowel Disease (IBD), particularly to differentiate between Crohn's disease and ulcerative colitis.
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
335
Bacterial Flora of the Large Intestine01:29

Bacterial Flora of the Large Intestine

461
The gut microbiome is formed by a vast and diverse community of bacteria that colonizes our large intestine. These bacteria start residing in the gut from birth and continue diversifying throughout life, influenced by factors such as diet, lifestyle, and stress. The gut bacterial community also includes bacteria from food and those that enter the colon through the anus.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
461
Anatomy of the Intestines01:23

Anatomy of the Intestines

72.1K
Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occurs in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
72.1K
Irritable Bowel Syndrome I: Introduction01:17

Irritable Bowel Syndrome I: Introduction

279
Irritable Bowel Syndrome (IBS) is characterized by functional disturbances in the gastrointestinal system, presenting a cluster of symptoms without evident structural or biochemical abnormalities. It primarily affects the large intestine and may cause abdominal pain, bloating, excessive gas, diarrhea, constipation, or both.
IBS is a chronic condition that can persist over a long period or recur frequently.
The pathogenesis of IBS involves a complex interplay of the following factors:
Altered...
279
What is Monogastric Digestion?01:50

What is Monogastric Digestion?

71.2K
The human body contains a monogastric digestive system. In a monogastric digestive system, the stomach only contains one chamber in which it digests food. Several other animal species also have monogastric digestive systems, including pigs, horses, dogs, and birds. This chapter, however, focuses on the human digestive system.
71.2K
Drugs Affecting GI Tract Motility: Antimicrobials as Antidiarrheal Agents01:18

Drugs Affecting GI Tract Motility: Antimicrobials as Antidiarrheal Agents

124
Acute diarrhea, a common gastrointestinal disturbance, is characterized by the rapid evacuation of fluid stools, leading to an excessive weight in fluid. This condition typically arises from disorders affecting intestinal water and electrolyte transport. It can be triggered by an increased osmotic load within the intestine, excessive secretion of electrolytes and water, mucosal exudation of protein and fluid, or altered intestinal motility. The primary risks of acute diarrhea are dehydration...
124

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Strain-level variation controls nutrient niche occupancy by health-associated <i>Anaerostipes hadrus</i>.

ISME communications·2025
Same author

Compositional analysis of bacterial peptidoglycan: insights from peptidoglycomics into structure and function.

Journal of bacteriology·2025
Same author

Members of Lachnospiraceae produce valerate and caproate in response to short-chain fatty acids.

Microbiome·2025
Same author

Long-chain unsaturated fatty acids released during immune responses stimulate host-microbe trans-kingdom communication.

Cell host & microbe·2025
Same author

Urease in acetogenic <i>Lachnospiraceae</i> drives urea carbon salvage in SCFA pools.

Gut microbes·2025
Same author

New opportunities in mechanistic and functional microbiome studies.

Cell chemical biology·2025

Related Experiment Video

Updated: Jul 8, 2025

An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota
07:15

An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota

Published on: July 31, 2019

9.6K

Fiber-deficient diets reprogram the microbiota.

Bradley G Fitzgerald1, Matthew T Sorbara1

  • 1Department of Molecular and Cellular Biology, the University of Guelph, 50 Stone Rd E, Guelph, ON N1G 2W1, Canada.

Cell Host & Microbe
|December 14, 2023
PubMed
Summary
This summary is machine-generated.

A fiber-deficient diet, used for pediatric Crohn's disease, thins the gut mucus layer. This diet change causes beneficial microbes to move away from the gut lining, reducing inflammation and improving colitis symptoms.

More Related Videos

A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model
08:14

A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model

Published on: February 28, 2018

8.9K
Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model
05:41

Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model

Published on: April 6, 2022

2.9K

Related Experiment Videos

Last Updated: Jul 8, 2025

An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota
07:15

An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota

Published on: July 31, 2019

9.6K
A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model
08:14

A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model

Published on: February 28, 2018

8.9K
Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model
05:41

Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model

Published on: April 6, 2022

2.9K

Area of Science:

  • Microbiome research
  • Gastroenterology
  • Pediatric Crohn's disease

Background:

  • Exclusive enteral nutrition (EEN) is a primary treatment for pediatric Crohn's disease.
  • EEN involves a fiber-deficient diet, but its precise effects on the gut microbiome and host-microbe interactions are not fully understood.

Purpose of the Study:

  • To investigate how a fiber-deficient diet impacts the gut mucus layer and microbial composition in the context of pediatric Crohn's disease.
  • To elucidate the mechanisms by which dietary fiber influences the gut environment and inflammatory responses.

Main Methods:

  • The study analyzed changes in the gut mucus layer thickness and microbial community structure in a model relevant to pediatric Crohn's disease.
  • Investigated microbial cross-feeding interactions and the spatial distribution of bacteria relative to the intestinal epithelium.

Main Results:

  • A fiber-deficient diet led to a significant thinning of the intestinal mucus layer.
  • Altered microbial cross-feeding patterns were observed, leading to a decrease in the abundance of pro-inflammatory bacteria, such as Mucispirillum, near the epithelium.
  • The observed changes resulted in the amelioration of colitis.

Conclusions:

  • Dietary fiber plays a crucial role in maintaining the integrity of the gut mucus layer.
  • Modulating microbial communities through diet can reduce inflammation and alleviate symptoms in pediatric Crohn's disease.
  • Findings provide insights into the therapeutic mechanisms of fiber-deficient diets in inflammatory bowel disease.