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Updated: Jul 8, 2025

Microscopy of Fission Yeast Sexual Lifecycle

Microscopy of Fission Yeast Sexual Lifecycle

Published on: March 9, 2016

Engineering heterothallic strains in fission yeast.

Daniel García-Ruano¹, Ian Hsu², Baptiste Leray¹

¹Institute of Biochemistry and Cellular Genetics, CNRS UMR 5095, University of Bordeaux, Bordeaux, France.

Yeast (Chichester, England)

|December 15, 2023

Summary

Researchers developed a simple CRISPR-Cas9 method to create heterothallic fission yeast strains. This technique efficiently engineers heterothallic cells from homothallic yeast, aiding genetic studies.

Keywords:

fission yeast heterothallic mating type engineering

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Area of Science:

Microbiology
Genetics
Molecular Biology

Background:

Schizosaccharomyces pombe (fission yeast) are homothallic, enabling self-mating and spore formation under nitrogen starvation.
Homothallic strains switch mating types (h- and h+) for efficient mating.
Heterothallic strains are crucial for Mendelian genetics research but are difficult to derive from natural homothallic isolates.

Purpose of the Study:

To develop an efficient and simple method for generating heterothallic fission yeast strains.
To overcome limitations of existing tedious or mutagenic strategies for creating heterothallic cells from homothallic parents.

Main Methods:

Utilized CRISPR-Cas9 gene editing technology.
Leveraged a previously identified alteration in the mating type locus of a switching-deficient strain.

Main Results:

Successfully generated heterothallic fission yeast strains in a single step.
Demonstrated high efficiency in engineering heterothallic cells from homothallic S. pombe.

Conclusions:

The described CRISPR-Cas9 approach provides a facile and efficient method for producing heterothallic fission yeast.
This advancement facilitates the use of diverse natural S. pombe isolates in genetic research.