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Updated: Jul 8, 2025
Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
Published on: March 19, 2018
Draft genomes of 12 Bifidobacterium isolates from human IBD fecal samples
Cole E Souza1, Nicole E Jacobson1, Michelle A An1
1Department of Biology, Viral Information Institute, San Diego State University , San Diego, California, USA.
Insights
This study isolated twelve Bifidobacterium strains from individuals with inflammatory bowel disease and healthy controls. The isolated species represent key Bifidobacterium species found in the human gut microbiome.
Area of Science:
- Microbiology
- Gastroenterology
- Human Gut Microbiome Research
Background:
- Inflammatory Bowel Disease (IBD) is associated with alterations in gut microbiota composition.
- Bifidobacterium species are crucial components of the human gut microbiome, known for their health benefits.
Purpose of the Study:
- To isolate and identify Bifidobacterium strains from fecal samples of IBD patients and healthy household controls.
- To characterize the Bifidobacterium species present in these distinct populations.
Main Methods:
- Fecal samples were collected from IBD patients and matched household controls.
- Bifidobacterium strains were isolated using selective culture techniques.
- Species identification was performed using standard microbiological methods.
Main Results:
- Twelve Bifidobacterium strains were successfully isolated.
- The isolated strains belonged to common Bifidobacterium species: B. adolescentis, B. animalis, B. breve, B. catenulatum, B. longum, and B. pseudocatenulatum.
Conclusions:
- This study provides a foundational list of Bifidobacterium species found in IBD patients and controls.
- Further research can utilize these isolates to investigate the role of specific Bifidobacterium species in IBD pathogenesis and potential therapeutic applications.
Abstract:
Twelve Bifidobacterium strains were isolated from fecal samples of inflammatory bowel disease patients and matched "household control" individuals. These include the species Bifidobacterium adolescentis, Bifidobacterium animalis, Bifidobacterium breve, Bifidobacterium catenulatum, Bifidobacterium longum, and Bifidobacterium pseudocatenulatum.
