Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multistep evolution from a metal-organic framework to ultrathin nanosheets.

Science bulletin·2023
Same author

A case report of autoimmune GFAP astrocytopathy presenting with abnormal heart rate variability and blood pressure variability.

BMC neurology·2023
Same author

Correction: Human Parvovirus B19 May Be a Risk Factor in Myasthenia Gravis with Thymoma.

Annals of surgical oncology·2022
Same author

The Role of Global and Local Ancestry on Clopidogrel Response in African Americans.

Pacific Symposium on Biocomputing. Pacific Symposium on Biocomputing·2022
Same author

ASO Author Reflections: A Possible New Etiology of Thymoma-Associated Myasthenia Gravis-Human Parvovirus B19.

Annals of surgical oncology·2022
Same author

Human Parvovirus B19 May Be a Risk Factor in Myasthenia Gravis with Thymoma.

Annals of surgical oncology·2022

Related Experiment Video

Updated: Jul 8, 2025

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

7.6K

Super-resolution stimulated Raman scattering microscopy enhanced by quantum light and deconvolution.

Li Gong, Shulang Lin, Zhiwei Huang

    Optics Letters
    |December 15, 2023
    PubMed
    Summary

    Researchers achieved quantum-enhanced super-resolution Stimulated Raman Scattering (SRS) microscopy using squeezed light and deconvolution. This technique significantly improves spatial resolution for label-free chemical imaging in biological samples.

    More Related Videos

    Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
    09:57

    Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

    Published on: July 25, 2022

    4.0K
    Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
    09:46

    Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

    Published on: April 28, 2022

    4.0K

    Related Experiment Videos

    Last Updated: Jul 8, 2025

    Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
    09:13

    Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

    Published on: July 6, 2019

    7.6K
    Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
    09:57

    Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

    Published on: July 25, 2022

    4.0K
    Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
    09:46

    Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

    Published on: April 28, 2022

    4.0K

    Area of Science:

    • Quantum optics
    • Microscopy
    • Biomedical imaging

    Background:

    • Stimulated Raman scattering (SRS) microscopy offers label-free chemical contrast bio-imaging.
    • Its capabilities are constrained by diffraction limits and shot noise inherent in light's quantum nature.

    Purpose of the Study:

    • To develop quantum-enhanced super-resolution SRS microscopy.
    • To overcome the diffraction limit and reduce noise in SRS imaging.

    Main Methods:

    • Utilized squeezed light generated via a novel cascaded nonlinear crystal scheme.
    • Applied a deconvolution method (Richardson-Lucy) in conjunction with squeezed light-controlled SRS.
    • Achieved shot noise suppression down to 89.7% (1.5 dB).

    Main Results:

    • Demonstrated a ~2.2-fold improvement in spatial resolution compared to conventional SRS.
    • Successfully implemented quantum-enhanced super-resolution SRS imaging in various samples, including oleic acid and porcine muscle tissue.

    Conclusions:

    • The combination of squeezed light and deconvolution provides a viable method for quantum-enhanced super-resolution SRS microscopy.
    • This approach holds significant potential for label-free super-resolution chemical imaging in biological and biomedical applications.