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Updated: Jul 8, 2025
Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma
Published on: October 14, 2016
Necrotizing apoptosis-related genes prognosis and treatment effect analysis of osteosarcoma in children
Yuanling Chen1, Ling Wu1, Yunyan Li1
1Ningbo Women and Children's Hospital, Ningbo, China.
Background:
Immune cell homeostasis plays a crucial role in cancer research and therapeutic response. While chemotherapy and immunotherapy hold promise in treating osteosarcoma (OS), identifying patients who are likely to respond would significantly improve clinical practices. Necroptosis, a fundamental mechanism mediating chemotherapy and immunotherapy efficacy, offers valuable insights. In this context, subtypes based on necroptosis-related genes have been established to predict the response of OS patients to immunotherapy and chemotherapy.
Methods:
We conducted a high-throughput screening test to identify necroptosis-associated genes that regulate the development of osteosarcoma. Subsequently, the ConsensusClusterPlus package was employed to classify OS patients into subtypes, enabling comparisons of prognosis and clinical information between these subtypes. Patients from the TARGET-OS and GSE21257 datasets were stratified into high-risk and low-risk groups, and their prognoses were compared. Additionally, we assessed the accuracy of the Risk Scoring Model in predicting prognosis, identified independent prognostic factors and explored potential chemotherapeutic agents and immunotherapy drugs.
Results:
Through the intersection of expression profiles from the TARGET-OS and GSE21257 datasets, we have identified a total of 92 genes associated with necroptosis. Based on differences in the expression of these genes, patients were divided into three subtypes, and we investigated the differences in tumor-infiltrating immune cells, immune-related pathways, and prognosis among these subtypes. Our nomogram effectively differentiated subtypes with distinct responses to chemotherapy and immunotherapy. The established signature demonstrated superior prediction ability compared with single clinical indicators.
Conclusions:
This pioneering study unveils the prognostic role of necroptosis-related genes in OS patients, providing a promising alternative for prognostic prediction in clinical disease management. Moreover, our findings highlight the significance of immune cell homeostasis in cancer research and therapeutic response, underscoring its relevance in advancing current treatment strategies.
Insights
Necroptosis-related genes can predict osteosarcoma patient response to chemotherapy and immunotherapy. This study identified subtypes based on these genes, improving prognostic prediction and guiding treatment strategies.
Area of Science:
- Cancer Research
- Immunology
- Genetics
Background:
- Immune cell homeostasis is vital for cancer research and treatment response.
- Chemotherapy and immunotherapy are promising for osteosarcoma (OS), but patient response prediction is needed.
- Necroptosis influences treatment efficacy and offers insights into patient stratification.
Purpose of the Study:
- To identify necroptosis-associated genes in osteosarcoma development.
- To classify OS patients into subtypes based on necroptosis gene expression for prognostic and therapeutic prediction.
- To evaluate a risk scoring model for predicting prognosis and identifying therapeutic targets.
Main Methods:
- High-throughput screening to identify necroptosis-associated genes.
- Consensus clustering to classify OS patients into subtypes using TARGET-OS and GSE21257 datasets.
- Prognostic analysis, risk scoring model development, and identification of independent prognostic factors.
Main Results:
- Identified 92 necroptosis-associated genes and classified OS patients into three subtypes.
- Subtypes showed distinct differences in immune cell infiltration, immune pathways, and prognosis.
- A nomogram-based signature effectively predicted chemotherapy and immunotherapy response, outperforming single clinical indicators.
Conclusions:
- Necroptosis-related genes play a significant prognostic role in osteosarcoma.
- This study provides a novel approach for prognostic prediction and clinical management of OS.
- Findings emphasize the importance of immune cell homeostasis in cancer therapy response.
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