Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to Membrane Traffic01:44

Introduction to Membrane Traffic

7.1K
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
7.1K
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

4.4K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
4.4K
Enlargement of the Plasma Membrane01:22

Enlargement of the Plasma Membrane

1.9K
Cell division and enlargement are processes that require precise control. The control ensures that cell division cannot proceed unless the cell has grown to a specific size. A spherical, dividing cell requires an approximately 1.6X increase in its surface area to double its volume. The secretory pathway also has a significant role in cell membrane enlargement. Secretory vesicles that bud off from the Golgi apparatus and later fuse with the plasma membrane during exocytosis are a major source of...
1.9K
Mechanisms of Membrane-bending01:15

Mechanisms of Membrane-bending

2.7K
The living membranes are flexible due to their fluid mosaic nature; however, their bending into different shapes is an active process regulated by specific lipids and proteins. The membrane bending can be transient as seen in vesicles or stable for a long time as in microvilli. Cells regulate the size, location, and duration of the membrane curvature.
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
2.7K
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

3.2K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
3.2K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

7.0K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
7.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A long-chain heparan sulfate capture mechanism directs paracrine GDNF-GFRα1 signalling through RET.

bioRxiv : the preprint server for biology·2026
Same author

Discovery of a sulfotyrosine-motif in the human TrkB extracellular domain required for agonist activation.

bioRxiv : the preprint server for biology·2026
Same author

RET receptor tyrosine kinase architecture, assemblies, and activation.

Endocrine-related cancer·2026
Same author

The chordoid glioma <i>PRKCA</i> D463H mutation is a kinase inactive, gain-of-function allele that induces early-onset chondrosarcoma in mice.

Science signaling·2025
Same author

Penetrant PKCβ mutation in ATLL displays a mixed gain-of-function.

The Biochemical journal·2025
Same author

Structural basis of Fanconi anemia pathway activation by FANCM.

The EMBO journal·2025

Related Experiment Video

Updated: Jul 8, 2025

Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies
10:22

Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies

Published on: July 13, 2013

19.4K

Into the fold: advances in understanding aPKC membrane dynamics.

Mathias Cobbaut1, Peter J Parker2,3, Neil Q McDonald1,4

  • 1Signalling and Structural Biology Laboratory, The Francis Crick Institute, NW1 1AT London, U.K.

The Biochemical Journal
|December 15, 2023
PubMed
Summary
This summary is machine-generated.

Atypical protein kinases C (aPKCs) are recruited to cell membranes through novel mechanisms, distinct from other protein kinases. This research clarifies how these key regulators of cell polarity are targeted to their functional sites.

Keywords:
apkcatypical pkccell polarityphospholipidsprotein kinase c

More Related Videos

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy
08:55

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy

Published on: February 17, 2023

3.1K
Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time
08:33

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Published on: March 11, 2021

2.0K

Related Experiment Videos

Last Updated: Jul 8, 2025

Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies
10:22

Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies

Published on: July 13, 2013

19.4K
Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy
08:55

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy

Published on: February 17, 2023

3.1K
Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time
08:33

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Published on: March 11, 2021

2.0K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Atypical protein kinase Cs (aPKCs) are crucial for cell polarity and are implicated in cancer.
  • aPKC localization to the plasma membrane is essential for their function.
  • The mechanisms of aPKC membrane recruitment were previously unclear.

Purpose of the Study:

  • To elucidate the mechanisms of atypical protein kinase C (aPKC) recruitment to the plasma membrane.
  • To integrate recent findings on aPKC membrane targeting with existing literature.
  • To provide insight into the regulation of aPKC in cellular processes.

Main Methods:

  • Literature review and synthesis of recent research findings.
  • Analysis of experimental data on protein-membrane interactions.
  • Comparative analysis with other protein kinase C (PKC) family members.

Main Results:

  • Recent studies reveal direct recruitment mechanisms for aPKCs to membranes.
  • These mechanisms differ from those of conventional and novel PKCs.
  • The findings help reconcile previously discrepant experimental observations.

Conclusions:

  • The direct recruitment of aPKCs to membranes is a key regulatory step.
  • Understanding these mechanisms is vital for comprehending cell polarization and cancer biology.
  • This work integrates diverse findings into a cohesive model of aPKC membrane targeting.