Quantitative source tracking for organic foulants in ultrafiltration membrane using stable isotope probing approach

Quantitatively identifying the primary sources of organic membrane fouling is essential for the effective implementation of membrane technology and optimal water resource management prior to the treatment. This study leveraged carbon stable isotope tracers to estimate the quantitative contributions of various organic sources to membrane fouling in an ultrafiltration system. Effluent organic matter (EfOM) and aquatic natural organic matter (NOM), two common sources, were combined in five different proportions to evaluate their mixed effects on flux decline and the consequent fouling behaviors. Generally, biopolymer (BP) and low molecular weight neutral (LMWN) size fractions - abundantly present in EfOM - were identified as significant contributors to reversible and irreversible fouling, respectively. Fluorescence spectroscopy disclosed that a protein-like component notably influenced overall membrane fouling, whereas humic-like components were predominantly responsible for irreversible fouling rather than reversible fouling. Fluorescence index (FI) and biological index (BIX), common fluorescence source tracers, showed promise in determining the source contribution for reversible foulants. However, these optical indices were insufficient in accurately determining individual source contributions to irreversible fouling, resulting in inconsistencies with the observed hydraulic analysis. Conversely, applying a carbon stable isotope-based mixing model yielded reasonable estimates for all membrane fouling. The contribution of EfOM surpassed 60 % for reversible fouling and increased with its content in DOM source mixtures. In contrast, aquatic NOM dominated irreversible fouling, contributing over 85 %, regardless of the source mixing ratios. This study emphasizes the potential of stable isotope techniques in accurately estimating the contributions of different organic matter sources to both reversible and irreversible membrane fouling.