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Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells
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Autophagy Gene BECN1 Intronic Variant rs9890617 Predisposes Individuals to Hepatitis B Virus Infection
Sargeet Kaur1, Jitendraa Vashistt1, Harish Changotra2
1Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan, Himachal Pradesh, 173 234, India.
Biochemical Genetics
|December 16, 2023
Summary
A specific gene variant, rs9890617 in BECN1, is linked to increased Hepatitis B virus (HBV) susceptibility. This finding suggests a genetic predisposition to HBV infection associated with this autophagy-related gene variant.
Area of Science:
- Genetics
- Virology
- Molecular Biology
Background:
- Hepatitis B virus (HBV) subverts host cell autophagy for replication.
- Beclin 1 (BECN1) is a key protein in the autophagy pathway, crucial for HBV multiplication.
Purpose of the Study:
- To investigate the association between BECN1 intronic variants and susceptibility to HBV infection.
- To analyze the functional impact of the rs9890617 variant on splicing signals.
Main Methods:
- Analysis of the rs9890617 intronic variant using Human Splicing Finder v3.1.
- Genotyping of 712 individuals (494 HBV-infected, 218 controls) using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP).
Main Results:
- The intronic variant rs9890617 was predicted to alter splicing signals.
- The mutant allele T of rs9890617 showed a significant association with overall HBV disease risk (OR 1.41, p=0.04).
- Significant associations were observed for the mutant genotype in chronic HBV infection across allelic, dominant, and co-dominant models.
Conclusions:
- The BECN1 variant rs9890617 is associated with genetic predisposition to HBV infection.
- The mutant T allele of rs9890617 may contribute to HBV susceptibility, particularly in chronic infection stages.
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