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Updated: Jul 8, 2025
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
Published on: September 25, 2019
Protocol for generation of humanized HCC mouse model and cancer-driver mutations using CRISPR-Cas9
Yicheng Zhu1, Stanley M Tahara1, Hidekazu Tsukamoto2
1Departments of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, Los Angeles, CA, USA.
Abstract:
We detail procedures for generating a humanized mouse model of hepatocellular carcinoma (HCC) recapitulating genetic mutations associated with metabolic liver diseases (MLD). We humanized liver parenchymal, non-parenchymal, and hematopoietic cells. We employed CRISPR-Cas9-based ARID1A knockout and constitutively active CTNNB1 knockin combined with an alcohol Western diet to generate cancer-driver mutations commonly found in MLD-HCC patients. This HCC model facilitates the study of tumor-promoting gene-environment interactions. For complete details on the use and execution of this protocol, please refer to Yeh et al.1.
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