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AMP-activated protein kinase (AMPK) suppresses Ibaraki virus propagation

Kiichi Ohkubo1, Shusaku Shibutani1, Hiroyuki Iwata1

  • 1Laboratory of Veterinary Hygiene, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, 753-8515, Japan.

Virology
|December 16, 2023
PubMed

Insights

Ibaraki virus (IBAV) propagation is suppressed by inhibiting mitochondrial ATP synthesis. AMP-activated protein kinase (AMPK) activation by these inhibitors, and during IBAV infection, suggests it is a key factor in controlling Ibaraki disease.

Area of Science:

  • Virology
  • Cell Biology
  • Biochemistry

Background:

  • Ibaraki virus (IBAV) causes significant disease in cattle.
  • IBAV infection involves macropinocytosis and endosomal escape, triggered by acidification.
  • Understanding IBAV replication mechanisms is crucial for disease control.

Purpose of the Study:

  • To investigate the role of mitochondrial oxidative phosphorylation in IBAV replication.
  • To identify host factors involved in IBAV propagation and suppression.
  • To explore potential therapeutic targets for Ibaraki disease.

Main Methods:

  • Treatment of infected cells with mitochondrial inhibitors (CCCP, antimycin A).
  • Measurement of cellular ATP levels and ATP synthesis.
  • Analysis of AMP-activated protein kinase (AMPK) activation.
  • Monitoring of IBAV propagation in treated and infected cells.

Main Results:

  • Inhibitors of mitochondrial oxidative phosphorylation significantly suppressed IBAV propagation.
  • Suppression of propagation correlated with reduced cellular ATP levels.
  • CCCP and antimycin A activated AMPK, which was also activated during IBAV infection.
  • IBAV infection itself led to ATP depletion and increased AMPK activity.

Conclusions:

  • Mitochondrial ATP synthesis is essential for efficient Ibaraki virus replication.
  • AMP-activated protein kinase (AMPK) plays a critical role in suppressing IBAV propagation.
  • AMPK activation represents a potential therapeutic strategy for managing Ibaraki disease in cattle.
Keywords:
AMPKATPIbaraki virusMitochondriaOrbivirus

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