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Related Concept Videos

High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

1.9K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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Types Of Column Chromatography01:29

Types Of Column Chromatography

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The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
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High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

578
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
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Principles Of Column Chromatography01:13

Principles Of Column Chromatography

6.8K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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Updated: Jul 8, 2025

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

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Automated method development in high-pressure liquid chromatography.

Emery Bosten1, Alexander Kensert2, Gert Desmet3

  • 1Department for Pharmaceutical and Pharmacological Sciences, Pharmaceutical Analysis, University of Leuven (KU Leuven), Herestraat 49, Leuven 3000, Belgium; Department of Pharmaceutical Development and Manufacturing Sciences, Janssen Pharmaceutica, Turnhoutseweg 30, Beerse, Belgium.

Journal of Chromatography. A
|December 17, 2023
PubMed
Summary
This summary is machine-generated.

Automating liquid chromatography method development streamlines complex separations. This review explores methods to remove human decision-making for faster, efficient analytical processes.

Keywords:
Active learningAutomationModellingSeparation optimization

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Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Method development in liquid chromatography is critical but challenging, requiring significant time, resources, and expertise.
  • Complexity and cost often hinder efficient analytical separation optimization.

Purpose of the Study:

  • To review methods for fully automated liquid chromatography method development.
  • To remove human intervention from the decision-making process in analytical separations.

Main Methods:

  • Discusses mathematical descriptions of separation problems using multi-criteria optimization.
  • Presents experimental and model-based strategies for optimization.
  • Reviews automated peak detection and tracking for closed-loop systems.

Main Results:

  • Highlights recent increases in interest for automated approaches.
  • Examines current methods, trends, shortcomings, and future prospects.
  • Demonstrates potential for facilitating, streamlining, and accelerating method development.

Conclusions:

  • Automated method development in liquid chromatography offers significant advantages.
  • Integration of reviewed methods can lead to a completely closed-loop process.
  • Future prospects focus on further enhancing efficiency and accessibility.