Continuous production of high-concentrated ammonia broth through fermentation

Being considered as a valuable resource and energy carrier, extensive research is going on to efficiently extract ammonia (NH₃) from anaerobic digestate. However, due to the well-known NH₃ inhibition on methanogens, the total NH₃ nitrogen (TAN) concentration is typically limited to 1-4 g N/L in digestate, making the NH₃ extraction process energy-consumptive. Here, NH₃ fermentation, specifically targeting augmented NH₃ production through biological reaction, was performed in a continuous mode. With the increase of gelatin input (10 to 150 g COD/L), NH₃ concentration and volumetric productivity gradually increased, reaching 12.0 g TAN-N/L and 36.0 g NH₃-N/L/d, which were the highest values ever reported. The stepwise increase in NH₃ exposure prompted a shift in microbial dominance towards Hathewaya (from 1 % to 68 %), a critical factor for having high NH₃ tolerance. Finally, NH₃ stripping results suggested that highly concentrated broth could reduce the specific energy consumption for NH₃ extraction to 1/3.