Related Experiment Video
Updated: Jul 8, 2025
11:13
Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
Published on: December 1, 2015
37.0K
Demonstration of direct immunofluorescence on three substrates in a case of pemphigus
Geethanjali Sahadevan1, Lissy Skaria2, Mary Vineetha1
1Department of Dermatology, Government Medical College, Kottayam, Kerala, India.
Pediatric Dermatology
|December 18, 2023
Abstract:
Direct immunofluorescence (DIF) on skin is considered as the gold standard in the diagnosis of pemphigus. However, alternate substrates can be used. We demonstrate DIF on three substrates, skin biopsy specimen, anagen hair and scrapings of oral erosions. Collection of alternative substrates can be more acceptable to young patients as it is less invasive. It may also be used to detect relapses in cases of pemphigus.
Related Concept Videos
01:12Immunofluorescence Microscopy
10.5K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
10.5K
01:20Immunogold Electron Microscopy
4.0K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
4.0K