Related Experiment Video
Updated: Jul 8, 2025
10:21
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
Published on: February 21, 2018
10.0K
Nuclear VANGL2 Inhibits Lactogenic Differentiation.
Biorxiv : the Preprint Server for Biology
|December 18, 2023
Summary
Planar cell polarity protein VANGL2 enters the nucleus and directly binds DNA, regulating mammary cell differentiation. Loss of VANGL2 function promotes differentiation by upregulating STAT5 signaling pathways.
Area of Science:
- Cell Biology
- Developmental Biology
- Molecular Biology
Background:
- Planar cell polarity (PCP) proteins regulate cell patterning and tissue morphogenesis.
- Intracellular roles of PCP proteins, including transcriptional control, remain largely unknown.
Approach:
- Investigated nuclear localization of the transmembrane PCP protein VANGL2 in HC11 cells, a model for mammary lactogenic differentiation.
- Utilized CUT&RUN assays to identify direct DNA binding of VANGL2 to specific motifs, including the Stat5a promoter.
- Examined the effects of Vangl2 knockdown and overexpression on mammary cell differentiation and gene expression.
Key Points:
- VANGL2 localizes to the nucleus in undifferentiated HC11 cells.
- Loss of Vangl2 function upregulates STAT5 signaling pathways and promotes mammary cell differentiation.
- VANGL2 directly binds to DNA motifs, including the Stat5a promoter, suggesting a transcriptional regulatory role.
Conclusions:
- PCP proteins, like VANGL2, can function intracellularly to regulate gene transcription.
- VANGL2 acts as a transcriptional repressor, keeping differentiation programs in check.
- These findings reveal a novel mechanism by which PCP proteins coordinate tissue morphogenesis through transcriptional control.
Related Concept Videos
01:14Introduction to Nuclear Reprogramming
1.9K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
1.9K
02:09Operons
49.1K
Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by...
49.1K
01:24Methods of Nuclear Reprogramming
1.8K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.8K
01:52Eukaryotic Transcription Inhibitors
9.8K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
9.8K
02:23Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K