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Updated: Jul 8, 2025

Measuring Spinal Presynaptic Inhibition in Mice By Dorsal Root Potential Recording In Vivo
Published on: March 29, 2014
GABA has a presynaptic inhibitory effect at Lumbricus terrestris body wall muscle synapses
William L Coleman1, Leah E McCartney1
1Biology, Commonwealth University of Pennsylvania- Bloomsburg. Bloomsburg, PA, USA.
Abstract:
Earthworm body wall muscle synapses have been suggested to contain both excitatory and inhibitory inputs, and therefore allow for investigation of excitatory/inhibitory signaling in an easily accessible model system. While previous studies have focused on postsynaptic GABAergic inhibitory mechanisms, this study investigated the hypothesis that GABAergic signaling also has presynaptic inhibitory function. This hypothesis was tested by loading synaptogreen C4 dye (also called FM1-43) into presynaptic vesicles in the presence of GABA at Lumbricus terrestris longitudinal muscle synapses. GABA treatment significantly reduced the fluorescence intensity observed at these synapses, suggesting that GABAergic signaling does indeed have a presynaptic inhibitory mechanism.
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