Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiome Profiling Reveals Astrocyte and Neuroendocrine Targets of Prenatal Acoustic Programming in Zebra Finch Embryos.

bioRxiv : the preprint server for biology·2026
Same author

Correction: Deep brain stimulation for Parkinson's disease in India: an expert consensus on availability, affordability, and eligibility by the Parkinson's research alliance India (PRAI).

Journal of neural transmission (Vienna, Austria : 1996)·2026
Same author

Modeling genetic mosaicism of the mammalian target of rapamycin pathway in the cerebral cortex.

Frontiers in mammal science·2026
Same author

Conserved mechanisms of plant lipidome remodeling under heat and cold stresses revealed through a systematic review and meta-analysis.

Journal of experimental botany·2026
Same author

Remote Language Assessment in School-Age Children With Phelan-McDermid Syndrome and Genotype-Phenotype Correlation.

American journal of medical genetics. Part A·2026
Same author

Deep brain stimulation for Parkinson's disease in India: an expert consensus on availability, affordability, and eligibility by the Parkinson's research alliance India (PRAI).

Journal of neural transmission (Vienna, Austria : 1996)·2026
Same journal

Advancing microalgae biomass cultivation for an integrated sustainable wastewater treatment and resource recovery.

iScience·2026
Same journal

Corrigendum to "Human adipose ECM alleviates radiation-induced skin fibrosis via endothelial cell-mediated M2 macrophage polarization" [iScience, Volume 26, Issue 9 (2023) 107660].

iScience·2026
Same journal

High-definition transcranial direct current stimulation enhances exercise-induced hypoalgesia in patients with chronic low back pain.

iScience·2026
Same journal

From pre-tumor to tumor: Decoding the endoscopic-pathologic spectrum of neoplastic lesions in autoimmune gastritis.

iScience·2026
Same journal

Corrigendum to "A cobalt-aluminium layered double hydroxide with a nickel core-shell structure nanocomposite for supercapacitor applications" [iScience, 28 (2025) 111672].

iScience·2026
Same journal

Repurposing primaquine diphosphate for imatinib-resistant chronic myeloid leukemia via targeting BCR-ABL and Wnt/β-catenin pathway.

iScience·2026
See all related articles

Related Experiment Video

Updated: Jul 8, 2025

Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases
09:36

Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases

Published on: June 28, 2019

10.0K

Tsc2 coordinates neuroprogenitor differentiation.

Victoria A Riley1, Vijay Shankar2,3, Jennie C Holmberg1

  • 1Department of Biological Sciences, Clemson University, Clemson, SC, USA.

Iscience
|December 18, 2023
PubMed
Summary
This summary is machine-generated.

Loss of Tsc2 in neural stem cells disrupts translation regulation, delaying differentiation and causing brain hamartomas. This highlights Tsc2

Keywords:
Cell biologyMolecular biologyMolecular mechanism of gene regulationNeuroscienceStem cells researchTranscriptomics

More Related Videos

GM-Free Generation of Blood-Derived Neuronal Cells
08:11

GM-Free Generation of Blood-Derived Neuronal Cells

Published on: February 13, 2021

3.0K
Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
10:47

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Published on: October 28, 2011

15.4K

Related Experiment Videos

Last Updated: Jul 8, 2025

Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases
09:36

Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases

Published on: June 28, 2019

10.0K
GM-Free Generation of Blood-Derived Neuronal Cells
08:11

GM-Free Generation of Blood-Derived Neuronal Cells

Published on: February 13, 2021

3.0K
Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
10:47

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Published on: October 28, 2011

15.4K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • Neural stem cells (NSCs) in the ventricular-subventricular zone (V-SVZ) generate diverse cell types.
  • Regulation of mRNA translation is crucial for cell differentiation, with the mTORC1 pathway playing a key role.
  • Somatic mutations in mTORC1 regulators, like TSC1/TSC2, are linked to brain abnormalities, including tuberous sclerosis complex (TSC).

Purpose of the Study:

  • To investigate the role of Tsc2 in V-SVZ neural stem cell differentiation and brain development.
  • To determine the impact of Tsc2 inactivation on mRNA translation and cell phenotype.
  • To elucidate the mechanism by which Tsc2 regulates translational control during differentiation.

Main Methods:

  • Inactivation of the Tsc2 gene in V-SVZ NSCs in vivo.
  • Analysis of translation factors, translatomes, and translational efficiency.
  • Single nuclei RNA sequencing to assess NSC activation states and differentiation.
  • Histological examination for brain hamartoma formation.

Main Results:

  • Tsc2 inactivation in V-SVZ NSCs led to the formation of striatal hamartomas.
  • Loss of Tsc2 altered translation factors, translatomes, and translational efficiency.
  • Impaired ability to decouple mRNA availability from translation resulted in delayed differentiation and retention of immature phenotypes.

Conclusions:

  • Tsc2 is essential for regulating translational repression during neural stem cell differentiation.
  • Disruption of Tsc2 function impairs the decoupling of mRNA translation, leading to developmental defects.
  • These findings establish Tsc2 as a critical regulator of differentiation and translational control in the brain.