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Related Experiment Video

Updated: May 5, 2026

Rapid Characterization of Genetic Parts with Cell-Free Systems

Rapid Characterization of Genetic Parts with Cell-Free Systems

Published on: August 30, 2021

Universal DNA-Based Sensing Toolbox for Programming Cell Functions.

Pei-Qiang Ma¹, Fu-Wen Huang¹, Ya-Qi Xie¹

¹Lab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology, Shanghai 200237, China.

Journal of the American Chemical Society

|December 18, 2023

Summary

Researchers developed a universal DNA sensing toolbox to enhance engineered cells and bacteria for therapies. This nanomechanical-natural hybrid system expands cell signaling capabilities for improved diagnosis and treatment applications.

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Published on: February 24, 2023

Area of Science:

Synthetic Biology
Nanotechnology
Cellular Engineering

Background:

Genetically engineered cells and bacteria offer innovative therapeutic potential.
Natural cell signaling pathways have limitations for advanced reprogramming.
Existing methods lack the specificity and efficacy for complex cellular control.

Purpose of the Study:

To develop a universal DNA-based sensing toolbox for cell surface engineering.
To overcome limitations of natural signaling pathways for enhanced cellular functions.
To create nanomechanical-natural hybrid cells for novel therapeutic strategies.

Main Methods:

Designed a DNA origami framework with a triangular-prismatic shape.
Integrated a sensing core within the framework to enhance specificity and efficacy.
Utilized customizable sensing cores with signal switches and converters for diverse inputs.

Main Results:

Demonstrated recognition of unconventional signal inputs.
Successfully delivered functional components to cells, controlling cell states.
Achieved specific tumor cell death, immune cell modulation, and bacterial expression.

Conclusions:

The DNA sensing toolbox expands the diversity of cell-sensed signals.
Nanomechanical-natural hybrid cells enable reprogramming of biological functions.
This approach provides new strategies for engineering cells and bacteria in diagnostics and therapeutics.