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PCR Analysis to Identify AAT Gene Promoters and Splice Variants

Noor Kalsheker¹

¹University of Nottingham, Nottingham, UK. noor.kalsheker@nottingham.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)

|December 18, 2023

Abstract:

This chapter explores the methods used for the analysis of alpha1-antitrypsin gene expression. This includes the use of the polymerase chain reaction (PCR), reverse transcriptase-PCR (RT-PCR), and whole transcriptome analysis combined with parallel DNA sequencing to understand the processes involved in AAT expression.

Keywords:

Alpha1-antitrypsin Alternative 5′ and 3′ transcripts PCR Reverse transcriptase- PCR SHAPE and MaP Tissue-specific expression

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