Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A coliphage lambda vector with enhanced biological containment: lambda gtALO.lambda B.

J M Tabor, V C Bode

    Gene
    |April 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Bovine viral diarrhea virus is inactivated when whole milk from persistently infected cows is heated to prepare semen extender.

    Veterinary microbiology·2008
    Same author

    Effects of ENU dosage on mouse strains.

    Mammalian genome : official journal of the International Mammalian Genome Society·2000
    Same author

    ENU-induced allele of brachyury (Tkt1) exhibits a developmental lethal phenotype similar to the original brachyury (T) mutation.

    The Journal of experimental zoology·1990
    Same author

    Pahhph-5: a mouse mutant deficient in phenylalanine hydroxylase.

    Proceedings of the National Academy of Sciences of the United States of America·1990
    Same author

    The use of N-ethyl-N-nitrosourea to produce mouse models for human phenylketonuria and hyperphenylalaninemia.

    Progress in clinical and biological research·1990
    Same author

    Genetic analysis of mouse t haplotypes using mutations induced by ethylnitrosourea mutagenesis: the order of T and qk is inverted in t mutants.

    Genetics·1988
    Same journal

    Face/off: phase-specific modeling of lineage plasticity using near-patient models in genitourinary cancers.

    Gene·2026
    Same journal

    Hierarchical analysis of metabolic phenotype reveals distinct microbiota and circulatory transcriptome in metabolic dysfunction-associated steatotic liver disease.

    Gene·2026
    Same journal

    Mutation T71R enhanced the structural stability and functional activity of wild type superoxide dismutase cloned from soil metagenome.

    Gene·2026
    Same journal

    Reduced ATXN1 expression as an adverse prognostic indicator in Acute myeloid leukemia.

    Gene·2026
    Same journal

    Constructing regulatory networks of Rubisco post-translational modifications: a novel avenue for engineering environment adaptive plants.

    Gene·2026
    Same journal

    Traumatic brain injury enhances fracture healing by upregulating VNN1 to activate the Wnt/β-catenin signaling pathway.

    Gene·2026
    See all related articles

    This study enhances biological containment for lambda gt cloning vectors by conditionally blocking DNA replication and phage assembly. This improves safety for genetic research and recombinant DNA preparation.

    Area of Science:

    • Molecular Biology
    • Microbiology
    • Biotechnology

    Background:

    • Lambda gt cloning vectors are widely used in molecular biology.
    • Enhancing biological containment is crucial for safe recombinant DNA technology.
    • Existing vectors may require improved safety features.

    Purpose of the Study:

    • To enhance the biological containment of lambda gt cloning vectors.
    • To develop a vector with conditional blocking of DNA replication and phage morphogenesis.
    • To evaluate the containment of a model recombinant vector for EK2 certification.

    Main Methods:

    • Construction of the lambda gtALO.lambda B vector by introducing specific mutations (Oam29, Aama1, Lam439).
    • Utilizing suppressor strains (suII+, suIII+) to control gene expression and phage development.

    Related Experiment Videos

  • Creation of a model recombinant vector (lambda gtALO.KmR') for containment assessment.

    • Main Results:

    • The Oam29 mutation conditionally blocks DNA replication, suppressed by suII+ or suIII+.
    • The Aama1 (head) and Lam439 (tail) mutations allow conditional control over phage assembly.
    • The constructed lambda gtALO.KmR' vector demonstrated enhanced biological containment properties.

    Conclusions:

    • The developed lambda gt vector system offers improved biological containment through conditional control of replication and morphogenesis.
    • This enhanced containment is valuable for safe preparation of large DNA quantities from individual isolates.
    • The vector's containment was validated through experiments for EK2 certification, indicating its safety potential.