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Nested Biofabrication: Matryoshka-Inspired Intra-Embedded Bioprinting
Mecit Altan Alioglu1,2, Yasar Ozer Yilmaz1,2,3, Yogendra Pratap Singh1,2
1The Huck Institutes of the Life Sciences, Penn State University, University Park, PA, 16802, USA.
Small Methods
|December 19, 2023
Summary
Intra-Embedded Bioprinting (IEB) fabricates complex organs with intricate internal structures. This novel bio-fabrication method utilizes a xanthan gum ink and support bath for precise organ modeling and drug delivery applications.
Area of Science:
- Bio-fabrication
- Tissue Engineering
- Bioprinting
Background:
- Engineering functional tissues and organs is crucial for regenerative medicine.
- Creating complex organ structures with integrated vasculature and innervation presents a significant challenge in bio-fabrication.
Purpose of the Study:
- To introduce a novel bio-fabrication method, Intra-Embedded Bioprinting (IEB), for creating complex organ structures.
- To demonstrate the capability of IEB in modeling organ features and developing functional tissue constructs.
Main Methods:
- Developed Intra-Embedded Bioprinting (IEB) using a xanthan gum-based ink with shear-thinning and self-healing properties.
- Utilized IEB to create a miniaturized pancreas replica, a head phantom, and a Matryoshka doll structure.
- Integrated extrusion-based and aspiration-assisted bioprinting for a breast tumor model with a vascular channel.
Main Results:
- Successfully fabricated intricate, nested structures and organ models, including a pancreas replica.
- Demonstrated the ability to create a breast tumor model with a central channel mimicking a blood vessel.
- Validated the efficacy of the tumor model for drug delivery, showing reduced tumor volume over time via perfusion.
Conclusions:
- Intra-Embedded Bioprinting (IEB) offers a new approach for bio-fabrication of complex organs with internal anatomical features.
- IEB enables precise organ modeling and the development of functional tissue constructs for applications like drug screening and regenerative medicine.
