Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material, molecules absorb light depending on the energy required for...

UV–Vis Spectrometers

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.

Spectrophotometry: Introduction

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Prolific S-layer shedding and associated proteins from the methanotroph <i>Methylomicrobium album</i> BG8.

Applied and environmental microbiology·2026

Same author

Diverse bacteriohemerythrin genes of <i>Methylomonas denitrificans</i> FJG1 provide insight into the survival and activity of methanotrophs in low oxygen ecosystems.

mBio·2025

Same author

Standardizing image acquisition and processing methods: a critical need for the accurate assessment of retinal blood vessel tortuosity.

Biomedical optics express·2025

Same author

Comparative study on the virulence of mycobacteriophages.

Journal of virology·2025

Same author

Retinal changes detected by diffuse reflectance spectroscopy in parkinsonian monkeys.

Neurophotonics·2025

Same author

Comparative study on the virulence of mycobacteriophages.

bioRxiv : the preprint server for biology·2024

Search research articles

Related Experiment Video

Updated: Jul 8, 2025

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

Published on: March 11, 2016

Targeted spectroscopy in the eye fundus.

Nicolas Lapointe¹, Cléophace Akitegetse¹, Jasmine Poirier¹

¹Zilia Inc., Quebec City, Québec, Canada.

Journal of Biomedical Optics

|December 19, 2023

Summary

Targeted ocular spectroscopy non-invasively assesses eye biomarkers for disease diagnosis. This study validates the technology in vitro and in vivo, demonstrating its potential for monitoring conditions like glaucoma and macular degeneration.

Keywords:

biomarkers diffuse reflectance fluorescence ocular spectroscopy oximetry

More Related Videos

Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration

Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration

Published on: May 26, 2023

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

Related Experiment Videos

Last Updated: Jul 8, 2025

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

Published on: March 11, 2016

Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration

Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration

Published on: May 26, 2023

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

Area of Science:

Ophthalmic diagnostics
Biomedical optics
Spectroscopy

Background:

Biomarker assessment in the eye is crucial for diagnosing and monitoring ocular and neurological diseases.
Targeted ocular spectroscopy offers a non-invasive method for analyzing eye fundus structure, composition, and function.

Purpose of the Study:

To demonstrate and validate the multimodal functionality of targeted ocular spectroscopy.
To assess its capabilities for in vitro and in vivo applications.

Main Methods:

Acquired and analyzed images and spectra from reference targets and a model eye.
Performed targeted ocular fluorescence spectroscopy.
Acquired in vivo diffuse reflectance spectra to assess blood oxygen saturation in healthy subjects.

Main Results:

Validated spectral analysis accuracy in specific imaging areas.
Observed distinct spectral signatures for different ocular regions in a model eye.
Demonstrated significant differences in blood oxygen saturation in vivo.

Conclusions:

Targeted ocular spectroscopy provides valuable structural, compositional, and functional information.
This technology enables new monitoring and diagnostic capabilities for eye diseases.
Potential applications include assessing oxygenation in glaucoma and diabetic retinopathy.