Related Experiment Video
Updated: Jul 8, 2025

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
Published on: May 12, 2023
Membrane contacts with the endoplasmic reticulum modulate plastid morphology and behaviour
Jaideep Mathur1, Thomas Kadanthottu Kunjumon1, Alena Mammone1
1Laboratory of Plant Development and Interactions, Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada.
Abstract:
Plastid behaviour often occurs in tandem with endoplasmic reticulum (ER) dynamics. In order to understand the underlying basis for such linked behaviour we have used time-lapse imaging-based analysis of plastid movement and pleomorphy, including the extension and retraction of stromules. Stable transgenic plants that simultaneously express fluorescent fusion proteins targeted to the plastid stroma, and the ER along with BnCLIP1-eGFP, an independent plastid envelope localized membrane contact site (MCS) marker were utilized. Our experiments strongly suggest that transient MCS formed between the plastid envelope and the ER are responsible for their concomitant behaviour.
Related Concept Videos
Plasmodesmata
Protein Transport to the Outer Chloroplast Membrane
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
Contact-dependent Signaling
Gap Junctions
In animal cells, gap junctions are formed...
Protein Transport to the Inner Chloroplast Membrane
Mechanisms of Membrane-bending
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
The Phragmoplast
The...

