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RESPIRATORY MICROBIOME AND ITS RELATIONSHIP WITH INFLAMMATORY MARKERS
Olesya M Horlenko1, Iryna Yu Pikina1, Lyubomyra B Prylypko1
1UZHHOROD NATIONAL UNIVERSITY, UZHHOROD, UKRAINE.
Wiadomosci Lekarskie (Warsaw, Poland : 1960)
|December 19, 2023
Summary
This study found Gram-negative bacteria dominate the nasopharyngeal microbiome in children with acute respiratory infections (ARI). This bacterial shift correlates with increased inflammatory markers and lactoperoxidase enzyme activity.
Area of Science:
- Microbiology
- Immunology
- Pediatrics
Background:
- Acute Respiratory Infections (ARI) are common in children.
- The nasopharyngeal microbiome plays a role in respiratory health and disease.
- Understanding the relationship between nasopharyngeal microflora and inflammation in ARI is crucial.
Purpose of the Study:
- To investigate the nasopharyngeal microbiome composition in children with ARI.
- To analyze the correlation between nasopharyngeal microflora and inflammatory markers in ARI patients.
- To compare the nasopharyngeal microbiome and inflammatory profiles of ARI patients with a healthy control group.
Main Methods:
- A case-control study involving 112 children with ARI and 25 healthy children.
- Analysis of nasopharyngeal microflora composition, identifying bacterial strains.
- Biochemical examination of biomaterial for enzyme and alcohol presence.
- Measurement of inflammatory markers including immunoglobulins (IgM, IgG, IgE), cytokines (IL-1, IL-4, IL-6, γ-IFN, TNF-α), Neopterin, and Thyroid Stimulating Hormone (TSH).
Main Results:
- Gram-negative bacteria predominated (71.0%) in the nasopharyngeal microflora of ARI patients, with Escherichia coli being prevalent (37.0%).
- Elevated levels of inflammatory markers were observed, including a 2.2-fold increase in IgM, a 10-fold increase in IgG, and higher levels of IL-1, IL-4, IL-6, γ-IFN, TNF-α, and Neopterin.
- The lactoperoxidase enzyme was predominantly detected in ARI patients, and TSH levels showed significant differences compared to the control group.
Conclusions:
- A shift towards Gram-negative bacteria in the nasopharyngeal microbiome is associated with ARI in children.
- Elevated inflammatory markers and lactoperoxidase enzyme predominance are characteristic of the nasopharyngeal environment in children with ARI.
- These findings highlight the complex interplay between the nasopharyngeal microbiome, inflammation, and acute respiratory infections.
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