Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

FTO Controls Endometrial Receptivity and Embryo Implantation through Regulating m<sup>6</sup>A and H3K27me3.

Communications biology·2026

Same author

Ythdf2/Setd1b regulatory axis is essential for cerebellar development through regulating epigenetic reprogramming.

Molecular psychiatry·2026

Same author

METTL3 Affects Endometrial Receptivity Through Modulation of P62-Mediated Autophagic Flux.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025

Same author

Fto-mediated m<sup>6</sup>A modification is essential for cerebellar development through regulating epigenetic reprogramming.

Journal of biomedical science·2025

Same author

Mettl3/Eed/Ythdc1 regulatory axis controls endometrial receptivity and function.

Communications biology·2025

Same author

The N6-methyladenosine landscape of ovarian development and aging highlights the regulation by RNA stability and chromatin state.

Aging cell·2024

Search research articles

Related Experiment Video

Updated: Jul 8, 2025

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

A low-cost, low-input method establishment for m6A MeRIP-seq.

Wenjuan Xia¹, Ling Guo¹, Huapeng Su¹

¹State Key Laboratory of Reproductive Medicine and Offspring Health, Suzhou Affiliated Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University, Suzhou, 215002, China.

Bioscience Reports

|December 19, 2023

Summary

This summary is machine-generated.

A new study shows CST's anti-m6A antibody effectively maps N6-methyladenosine (m6A) RNA modifications using MeRIP-seq. This cost-effective alternative to the discontinued Millipore antibody provides similar results at lower doses, enabling large-scale m6A transcriptome analysis.

Keywords:

CST MeRIP-seq alternative splicing anti-m6A antibody m6A

More Related Videos

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

Related Experiment Videos

Last Updated: Jul 8, 2025

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

Area of Science:

Molecular Biology
Epigenetics
Transcriptomics

Background:

N6-methyladenosine (m6A) is a prevalent mRNA modification regulating cellular functions.
m6A RNA immunoprecipitation sequencing (MeRIP-seq) is crucial for identifying m6A locations.
The widely used Millipore MABE572 anti-m6A antibody is expensive and discontinued.

Purpose of the Study:

To evaluate CST's anti-m6A antibody as a cost-effective and accessible alternative for MeRIP-seq.
To compare the efficacy of CST antibody-based MeRIP-seq with the MABE572 antibody.
To optimize MeRIP-seq protocols for large-scale m6A transcriptome mapping.

Main Methods:

Performed MeRIP-seq using varying concentrations of CST anti-m6A antibody with HEK293T cell RNA.
Compared m6A peak calling, motif enrichment, and alternative splicing events between CST and Millipore libraries.
Analyzed nuclear transcripts modified by m6A using both antibody types.

Main Results:

CST antibody-based MeRIP-seq yielded comparable data to the Millipore MABE572 antibody.
Effective m6A mapping was achieved even with significantly reduced amounts of CST antibody.
The CST antibody significantly lowers the cost and volume requirements for MeRIP-seq.

Conclusions:

CST's anti-m6A antibody is a viable and economical replacement for MABE572 in MeRIP-seq.
This refined MeRIP-seq protocol facilitates large-scale m6A transcriptome analysis.
Reduced antibody usage and cost make m6A mapping more accessible for researchers.