Iron cycle-enhanced anaerobic ammonium oxidation in microaerobic granular sludge

Granule-based partial nitritation and anaerobic ammonium oxidation (PN/A) is an energy-efficient approach for treating ammonia wastewater. When treating low-strength ammonia wastewater, the stable synergy between PN and anammox is however difficult to establish due to unstable dissolved oxygen control. Here, we proposed, the PN/A granular sludge formed by a micro-oxygen-driven iron redox cycle with continuous aeration (0.42 ± 0.10 mg-O₂/L) as a novel strategy to achieve stable and efficient nitrogen (N) removal. 240-day bioreactor operation showed that the iron-involved reactor had 37 % higher N removal efficiency than the iron-free reactor. Due to the formation of the microaerobic granular sludge (MGS), the bio(chemistry)-driven iron cycle could be formed with the support of anaerobic ammonium oxidation coupled to Fe³⁺ reduction. Both ammonia-oxidizing bacteria and generated Fe²⁺ could scavenge the oxygen as a defensive shield for oxygen-sensitive anammox bacteria in the MGS. Moreover, the iron minerals derived from iron oxidation and Fe-P precipitates were also deposited on the MGS surface and/or embedded in the internal channels, thus reducing the size of the channels that could limit oxygen mass transfer inside the MGS. The spatiotemporal assembly of diverse functional microorganisms in the MGS for the realization of stable PN/A could be achieved with the support of the iron redox cycle. In contrast, the iron-free MGS could not optimize oxygen mass transfer, which led to an unstable and inefficient PN/A. This work provides an alternative iron-related autotrophic N removal for low-strength ammonia wastewater.