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GPATCH4 contributes to nucleolus morphology and its dysfunction impairs cell viability
Kazuki Kodera1, Ryuichi Hishida2, Akiko Sakai2
1Department of Neuroscience of Disease, Brain Research Institute, Niigata University, Niigata, 951-8585, Japan; Department of Paediatrics, Niigata University Graduate School of Medical and Dental Sciences, Niigata, 951-8510, Japan.
Biochemical and Biophysical Research Communications
|December 19, 2023
Summary
G-patch domain containing 4 (GPATCH4) is a nucleolar protein crucial for cell viability. Its dysfunction impairs cell proliferation, alters nucleolar structure, and affects cellular senescence and apoptosis.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- The nucleolus is vital for rRNA transcription, ribosome synthesis, cell cycle regulation, and senescence.
- G-patch domain containing 4 (GPATCH4) is a nucleolar protein with unclear functional significance.
Purpose of the Study:
- To elucidate the functions of GPATCH4 by examining the effects of its dysfunction on cellular proliferation, nucleolar architecture, apoptosis, and senescence.
Main Methods:
- Experiments were conducted on cultured neuroblastoma SH-SY5Y cells.
- GPATCH4 reduction/knockdown was induced.
- Cellular proliferation, apoptosis, nucleolar morphology, senescence markers (SA-β-GAL, p16), and ribosomal gene expression were analyzed.
Main Results:
- GPATCH4 reduction inhibited cellular proliferation and increased apoptosis susceptibility.
- Nucleolar morphology changed, with fewer but larger nucleoli.
- GPATCH4 knockdown decreased cell viability and increased senescence markers upon low-dose etoposide treatment.
- GPATCH4 dysfunction altered ribosomal system gene expression.
Conclusions:
- GPATCH4 is a pivotal nucleolar protein regulating nucleolar morphology.
- GPATCH4 is correlated with cell viability, proliferation, and senescence.
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