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Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
Published on: May 18, 2017
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Global quantitative proteomic analysis of aged mouse hippocampus.
He Huang1,2, Ashley J van Waardenberg3, Mark E Graham4
1Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, QLD, Australia.
Proteomics
|December 20, 2023
Summary
This study reveals key protein changes in the aged mouse hippocampus, showing increased immune and metabolic proteins but decreased neuron-specific proteins, offering insights into cognitive decline.
Area of Science:
- Neuroscience
- Proteomics
- Aging Research
Background:
- The aged brain exhibits molecular changes impacting cognition.
- The hippocampus is crucial for learning and memory, making it a key focus for aging studies.
Purpose of the Study:
- To investigate global proteomic alterations in the hippocampus of aged mice compared to young mice.
- To identify specific protein changes associated with normal brain aging.
Main Methods:
- Tandem mass tag (TMT) methodology was used for quantitative proteomic analysis.
- Hippocampal tissues from advanced-aged (20-month) and young (3-month) C57BL/6 male mice were analyzed.
Main Results:
- Upregulation of 236 proteins in aged hippocampus, enriched in adaptive immune and metabolic pathways.
- Downregulation of 88 proteins, primarily involved in axonogenesis and growth cone processes.
- Increased expression of proteins in microglia, astrocytes, and oligodendrocytes, contrasted by decreased neuron-specific proteins.
Conclusions:
- Proteomic analysis reveals distinct molecular shifts in the aged hippocampus.
- Age-related changes include altered glial cell protein expression and neuronal protein reduction.
- Correlation with transcriptomic data supports these findings for understanding aging mechanisms.
