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Updated: Jul 8, 2025

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy
Published on: January 19, 2011
Method of spectral response to stochastic processes for measuring the nonreciprocal effective interactions
E A Sametov1, E A Lisin1, O S Vaulina1
1Joint Institute for High Temperatures, 125412 Moscow, Russia and Moscow Institute of Physics and Technology, 125412 Moscow, Russia.
Abstract:
The theoretical background of the nonperturbative method of spectral response to stochastic processes (SRSP) for measuring the nonreciprocal interparticle effective interactions in strongly coupled underdamped systems is described. Analytical expressions for vibrational spectral density of confined Brownian particles with a nonreciprocal effective interaction are presented. The changes in the vibrational spectral density with varying different parameters of the system (nonreciprocity, viscosity, ratios of particle sizes, and intensities of random processes acting on each particle) are discussed using the example of a pair of nonidentical particles in a harmonic trap. The SRSP method is compared to three other nonperturbative methods. The SRSP method demonstrates an undeniable advantage when processing particle trajectories with errors in particle tracking.
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