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Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis
Published on: December 23, 2022
4.9K
Organoid assessment technologies.
Yuyuan Gu1,2,3, Wencai Zhang4, Xianmin Wu5
1Institute of Translational Medicine, Shanghai University, Shanghai, China.
Clinical and Translational Medicine
|December 20, 2023
Summary
Developing stable organoid models remains challenging. This review explores novel multi-omics and histological approaches to standardize organoid generation and analysis for improved human tissue resemblance and function.
Area of Science:
- Stem Cell Biology
- Tissue Engineering
- Biotechnology
Background:
- Organoid generation has advanced, yet robust and stable protocols are a significant challenge for researchers.
- Assessing organoid structure and function in vitro or in vivo is often limited by precision strategies.
- Standardizing organoid generation to accurately mimic human-derived tissue is a growing area of interest.
Purpose of the Study:
- To review novel approaches for studying organoid molecular mechanisms and cell heterogeneity.
- To discuss the histological and morphological similarities between organoids and human tissues.
- To explore future perspectives for functional analysis and the maturation of organoid models.
Main Methods:
- Multi-omics analysis to decipher cell-cell interaction mechanisms.
- Single-cell analysis for detailed investigation of cellular components.
- Histological analysis for evaluating organoid structures and morphology.
Main Results:
- Novel strategies enable detailed investigation of organoid cell-cell interactions and structures.
- Multi-omics and single-cell analyses provide insights into molecular mechanisms and cell heterogeneity.
- Histological analysis allows for comparison of organoid morphology to human tissues.
Conclusions:
- Standardized organoid protocols are crucial for accurate human tissue modeling.
- Advanced analytical techniques like multi-omics and histology are key to understanding organoid complexity.
- Future research will focus on enhancing functional analysis to mature organoids as reliable models.
