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Surface protein of a Streptococcus agalactiae isolate
Infection and Immunity
|June 1, 1979
Summary
Researchers purified a cell surface protein antigen from bovine Streptococcus agalactiae. This protein, with a molecular weight of 31,800, is a potential target for diagnostics or vaccines against bovine mastitis.
Area of Science:
- Bacteriology
- Immunology
- Veterinary Medicine
Background:
- Streptococcus agalactiae is a significant pathogen in cattle.
- Identifying cell surface antigens is crucial for developing targeted interventions.
Purpose of the Study:
- To isolate and characterize a protein antigen from a bovine Streptococcus agalactiae isolate.
- To determine the antigen's location within the bacterial cell.
Main Methods:
- Bovine Streptococcus agalactiae cultured and radioiodinated.
- Cell extraction at low pH with nonionic detergent.
- Protein purification via ultracentrifugation, gel filtration, and ion-exchange chromatography.
Main Results:
- A protein antigen was successfully purified.
- The protein's molecular weight was estimated at 31,800 Daltons.
- Agglutinogenic properties confirmed cell surface localization.
Conclusions:
- A 31,800 MW protein antigen is located on the surface of bovine Streptococcus agalactiae.
- This antigen is a potential candidate for further investigation in vaccine development or diagnostic tools.