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Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
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Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane

Published on: February 8, 2020

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FOXM1/KIF20A axis promotes clear cell renal cell carcinoma progression via regulating EMT signaling and affects

Kai Fang1, Min Gong1, Dong Liu1

  • 1Department of Urology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, China.

Heliyon
|December 21, 2023
PubMed
Abstract

Insights

The FOXM1/KIF20A axis promotes clear cell renal cell carcinoma (ccRCC) progression and impacts immunotherapy response. Inhibiting this axis suppresses ccRCC malignancy and offers a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • The relationship between Forkhead box protein M1 (FOXM1) and Kinesin family member 20A (KIF20A) in clear cell renal cell carcinoma (ccRCC) remains underexplored.
  • Understanding novel molecular correlations is crucial for advancing ccRCC treatment strategies.

Purpose of the Study:

  • To elucidate the correlation between FOXM1 and KIF20A in ccRCC.
  • To investigate the functional role of the FOXM1/KIF20A axis in ccRCC progression and its impact on immunotherapy.

Main Methods:

  • Bioinformatic analysis of The Cancer Genome Atlas (TCGA) database.
  • Quantitative real-time PCR (qRT-PCR) and Western blot for gene and protein expression.
  • In vitro assays (CCK8, colony formation, Transwell) to assess cell growth, invasion, and migration.
  • Analysis of the epithelial-mesenchymal transition (EMT) signaling pathway.

Main Results:

  • FOXM1 and KIF20A expression are significantly associated with poor prognosis and clinical progression in ccRCC.
  • Inhibition of FOXM1 and KIF20A effectively suppresses ccRCC cell malignancy in vitro.
  • FOXM1 positively regulates KIF20A expression, and this axis activates EMT signaling.
  • The FOXM1/KIF20A axis influences the tumor immune microenvironment and immunotherapy response in ccRCC.

Conclusions:

  • The FOXM1/KIF20A axis plays a critical role in ccRCC progression and is implicated in modulating immunotherapy outcomes.
  • Targeting the FOXM1/KIF20A axis presents a promising therapeutic strategy for ccRCC treatment.
Keywords:
EMTFOXMImmunotherapyKIF20AccRCC

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