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Published on: March 30, 2019
Whole-genome microRNA sequencing analysis in patients with pulmonary hypertension
Shi Chen1,2, Jinnan Zhong2, Bingzhu Hu2
1Division of Respiratory and Critical Care Medicine, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China.
Abstract:
Pulmonary hypertension (PH) is a pathological disorder with multiple clinical manifestations that lead to cardiovascular and respiratory diseases in most patients. Recent studies have revealed that microRNAs (miRNAs) play important roles as upstream signaling molecules in several diseases, including PH. However, miRNAs that can be used as diagnostic or prognostic biomarkers for PH have not been identified. Thus, in this study, peripheral blood samples obtained from patients with PH and healthy individuals were subjected to genome-wide miRNA sequencing and transcriptome analysis. We screened 136 differentially expressed miRNAs in patients with PH and verified that four differentially expressed miRNAs, namely, hsa-miR-1304-3p, hsa-miR-490-3p, hsa-miR-11400, and hsa-miR-31-5p, could be used as clinical diagnostic biomarkers for pulmonary arterial hypertension. Our findings provide a basis for further in-depth investigations of the specific mechanisms of miRNAs in PH.
Insights
Researchers identified four microRNAs (miRNAs) in peripheral blood that can serve as diagnostic biomarkers for pulmonary arterial hypertension (PAH). This discovery offers potential for earlier detection and improved management of this cardiovascular and respiratory disease.
Area of Science:
- Biochemistry
- Genetics
- Cardiology
Background:
- Pulmonary hypertension (PH) is a complex condition affecting cardiovascular and respiratory systems.
- MicroRNAs (miRNAs) are increasingly recognized as key signaling molecules in disease pathogenesis.
- Reliable diagnostic and prognostic biomarkers for PH are currently lacking.
Purpose of the Study:
- To identify novel microRNA (miRNA) biomarkers for the diagnosis of pulmonary hypertension (PH).
- To investigate the potential of circulating miRNAs as non-invasive diagnostic tools for PH.
Main Methods:
- Genome-wide miRNA sequencing and transcriptome analysis of peripheral blood samples from PH patients and healthy controls.
- Screening for differentially expressed miRNAs.
- Validation of candidate miRNA biomarkers.
Main Results:
- Identified 136 differentially expressed miRNAs in patients with PH.
- Validated four specific miRNAs (hsa-miR-1304-3p, hsa-miR-490-3p, hsa-miR-11400, and hsa-miR-31-5p) as potential clinical diagnostic biomarkers for pulmonary arterial hypertension.
- These miRNAs show promise for distinguishing PH patients from healthy individuals.
Conclusions:
- The identified miRNAs represent potential novel diagnostic biomarkers for pulmonary arterial hypertension.
- These findings lay the groundwork for understanding the role of specific miRNAs in PH.
- Further research into the mechanistic roles of these miRNAs in PH is warranted.
