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CircSFMBT2 Plays an Oncogenic Role in Lung Adenocarcinoma Depending on the miR-1305/SALL4 Axis

Xuan Zhao1, Xiaojing Xing1, Yongkai Wu2

  • 1Department of Hematology and Breast Medicine, Cancer Hospital of China Medical University, No. 44, Xiaoheyan Road, Dadong District, Shenyang, 110801, Liaoning, China.

Biochemical Genetics
|December 21, 2023
PubMed

Insights

Circular RNA SFMBT2 (circSFMBT2) promotes lung adenocarcinoma (LUAD) progression by targeting the miR-1305/SALL4 pathway. This circRNA may serve as a potential diagnostic and prognostic marker for LUAD patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Circular RNAs (circRNAs) play critical roles in various cancers, including lung adenocarcinoma (LUAD).
  • The specific function of circRNA scm like with four mbt domains 2 (circSFMBT2) in LUAD remains largely unexplored.

Purpose of the Study:

  • To investigate the role and mechanism of circSFMBT2 in the progression of lung adenocarcinoma.
  • To explore circSFMBT2 as a potential diagnostic and prognostic biomarker for LUAD.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR), western blot, and immunohistochemistry (IHC) were used to assess molecule expression.
  • Cellular assays (EdU, transwell, flow cytometry) and a mouse xenograft model were employed to evaluate LUAD cell behaviors and tumor growth.
  • Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays were conducted to confirm molecular interactions.

Main Results:

  • CircSFMBT2 was found to be upregulated in LUAD tissues and correlated with advanced TNM stage and poor prognosis.
  • Knockdown of circSFMBT2 inhibited LUAD cell proliferation, metastasis, and glycolysis, while inducing apoptosis in vitro and suppressing tumor formation in vivo.
  • CircSFMBT2 directly targeted microRNA-1305 (miR-1305), and miR-1305 targeted spalt like transcription factor 4 (SALL4).
  • The circSFMBT2/miR-1305/SALL4 axis was identified as a key pathway driving LUAD progression.

Conclusions:

  • CircSFMBT2 promotes LUAD progression by sponging miR-1305 and upregulating SALL4.
  • CircSFMBT2 acts as an oncogene in LUAD and represents a potential diagnostic and prognostic biomarker.
Keywords:
Lung adenocarcinoma (LUAD)SALL4circSFMBT2miR-1305

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