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Placental cell conditioned media modifies hematopoietic stem cell transcriptome invitro.

Sean M Harris1, Anthony L Su2, John F Dou3

  • 1School of Public Health, Department of Environmental Health Sciences, University of Michigan, 1415 Washington Heights, Ann Arbor, MI, 48109, USA.

Placenta
|December 21, 2023
PubMed
Summary

Placental cell secretions influence hematopoietic stem cell gene expression, affecting pathways like stem cell maintenance. The environmental toxicant monoethylhexyl phthalate (MEHP) also impacts these cells, altering lipid metabolism genes.

Keywords:
Cell communicationHematopoieticPhthalatePlacentaTranscriptomic

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Area of Science:

  • Hematology
  • Toxicology
  • Cell Biology

Background:

  • Hematopoietic stem cells (HSCs) generate all blood cell types.
  • Placental factors are crucial for maternal/fetal health, but their communication with HSCs is understudied.
  • Toxicant effects on placental-HSC communication are largely unknown.

Purpose of the Study:

  • To investigate if placental cell secretions alter HSC transcriptomic responses.
  • To determine if monoethylhexyl phthalate (MEHP) modifies these placental-HSC interactions.
  • To explore the impact of environmental toxicants on stem cell communication.

Main Methods:

  • Used K-562 cells (HSC model) and BeWo cells (placental syncytiotrophoblast model) in vitro.
  • Treated K-562 cells with BeWo-conditioned medium, MEHP-treated BeWo-conditioned medium, or control medium.
  • Performed RNA sequencing and differential gene expression analysis on K-562 cells.

Main Results:

  • Placental conditioned medium altered 173 genes in K-562 cells, upregulating cytoskeletal and myeloid differentiation regulators.
  • Upregulated genes were enriched in stem cell maintenance, proliferation, and immune response pathways.
  • MEHP treatment altered 8 genes, including those involved in lipid metabolism.

Conclusions:

  • K-562 cells, modeling HSCs, respond to placental cell secretions, impacting stem cell maintenance pathways.
  • Placental-HSC communication is susceptible to environmental toxicant exposure.
  • Further research is needed to understand toxicant-mediated disruption of placental-HSC signaling.