Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Low-cost two-dimensional gel densitometry.

R M Levenson, E V Maytin, D A Young

    Analytical Biochemistry
    |November 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Singlet Molecular Oxygen: from COIL Lasers to Photodynamic Cancer Therapy.

    The journal of physical chemistry. B·2023
    Same author

    Highly efficient CRISPR-Cas9-mediated editing identifies novel mechanosensitive microRNA-140 targets in primary human articular chondrocytes.

    Osteoarthritis and cartilage·2022
    Same author

    Osteoarthritis year in review: genetics, genomics, epigenetics.

    Osteoarthritis and cartilage·2021
    Same author

    TimiRGeN: R/Bioconductor package for time series microRNA-mRNA integration and analysis.

    Bioinformatics (Oxford, England)·2021
    Same author

    Identification of long non-coding RNAs expressed in knee and hip osteoarthritic cartilage.

    Osteoarthritis and cartilage·2019
    Same author

    Nonrestorative Treatments for Caries: Systematic Review and Network Meta-analysis.

    Journal of dental research·2018
    Same journal

    Evaluation of a method for affinity measurement using solution equilibrium titration with magnetic beads.

    Analytical biochemistry·2026
    Same journal

    Metabolomics approach using UHPLC/QE-MS for the Mechanism of He Xue Ming Mu Tablets on Non-Proliferative Diabetic Retinopathy.

    Analytical biochemistry·2026
    Same journal

    UniRES-GO: Unified residue-level early fusion of sequence and predicted structure for protein function prediction.

    Analytical biochemistry·2026
    Same journal

    IgG detection by enzyme-linked mass spectrometric assay versus color, fluorescent, ECL in buffer and serum.

    Analytical biochemistry·2026
    Same journal

    A PCR-based assay for distinguishing between 293, 293T, and 293E cell lines.

    Analytical biochemistry·2026
    Same journal

    Real-time spectrophotometric microplate assay for galactolipase activity using synthetic or natural galactolipids in mixed micelles.

    Analytical biochemistry·2026
    See all related articles

    We developed GELSCAN, an affordable 2-D gel densitometer using a Commodore microcomputer. This system simplifies quantifying autoradiograms, offering excellent reproducibility for 2-D gel electrophoresis analysis.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Biotechnology

    Background:

    • Two-dimensional (2-D) gel electrophoresis is powerful but hindered by complex quantification.
    • Existing densitometry methods are often expensive and difficult to use.

    Purpose of the Study:

    • To develop a low-cost, user-friendly densitometer for 2-D gel electrophoresis.
    • To improve the accessibility and efficiency of quantifying gel electrophoresis results.

    Main Methods:

    • Utilized an analog-to-digital converter in Commodore 64/128 microcomputers.
    • Developed the GELSCAN system with a handheld photoelectric sensor (mouse).
    • Employed manual spot positioning, density determination, and internal calibration for quantification.

    Related Experiment Videos

    Main Results:

    • GELSCAN adds minimal cost ($20) to existing Commodore systems.
    • System quantifies autoradiograms, corrects for film nonlinearity, and allows background subtraction/normalization.
    • Demonstrated excellent reproducibility and versatility for 1-D gels and dot-blots.

    Conclusions:

    • GELSCAN offers a practical and cost-effective solution for 2-D gel electrophoresis densitometry.
    • The system simplifies data analysis and enhances reproducibility.
    • GELSCAN provides a valuable alternative to more complex and expensive quantification methods.