Related Experiment Video
Updated: Jul 7, 2025
08:25
Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
Published on: June 5, 2020
6.8K
Precision nephrotoxicity testing using 3D in vitro models
Pengfei Yu1,2, Hainan Zhu1, Carol Christine Bosholm1
1Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Winston-Salem, NC, USA.
Cell & Bioscience
|December 22, 2023
Summary
Three-dimensional (3D) in vitro models using human kidney cells offer a more accurate way to test drug safety and chemical toxicity. These advanced models better mimic the human kidney than traditional methods, improving nephrotoxicity predictions.
Area of Science:
- Nephrology
- Toxicology
- Biotechnology
Background:
- Nephrotoxicity is a critical safety concern in drug development and chemical assessment.
- Traditional testing methods, including animal models and 2D in vitro cultures, inadequately replicate human kidney complexity and function.
- Existing 2D models lack the physiological relevance needed for accurate human nephrotoxicity prediction.
Purpose of the Study:
- To review the application of 3D in vitro models for precision nephrotoxicity testing.
- To highlight the advantages of using human autologous urine-derived kidney cells in these models.
- To present 3D in vitro models as a promising approach for evaluating drug safety.
Main Methods:
- Utilizing 3D microenvironments to culture human primary kidney cells.
- Mimicking in vivo kidney conditions, including fluid shear stresses.
- Employing human autologous urine-derived kidney cells for model development.
Main Results:
- 3D in vitro models demonstrate superior accuracy and reliability in predicting human nephrotoxicity compared to 2D models.
- These models offer a more physiologically relevant platform for toxicity assessment.
- Autologous urine-derived cells provide a patient-specific approach to kidney modeling.
Conclusions:
- 3D in vitro models represent a significant advancement in nephrotoxicity testing.
- The use of human autologous urine-derived kidney cells enhances the precision and predictive power of these models.
- Precision nephrotoxicity testing with 3D models is a promising strategy for improving drug safety evaluations.
