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Sodium-sensitive glass microelectrode: modified Thomas recessed-tip configuration.

L R Pucacco, S K Corona, N W Carter

    Analytical Biochemistry
    |November 15, 1986
    PubMed
    Summary
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    A new modified Thomas electrode was developed to measure intracellular sodium concentration in epithelial cells. This highly sensitive and selective microelectrode offers precise sodium measurement for cellular research.

    Area of Science:

    • Biomedical Engineering
    • Cellular Physiology
    • Electrochemistry

    Background:

    • Accurate measurement of intracellular sodium concentration is crucial for understanding cellular function.
    • Existing methods for sodium measurement may have limitations in sensitivity or specificity.

    Purpose of the Study:

    • To develop a novel sodium-sensitive microelectrode for direct measurement of intracellular sodium in epithelial cells.
    • To characterize the performance of the modified Thomas electrode.

    Main Methods:

    • Development of a modified Thomas electrode utilizing a glass-membrane, sodium-sensitive microelectrode.
    • Characterization of electrode sensitivity, selectivity, electrical resistance, and sensing chamber volume.
    • Design for potential double-barreled configuration.

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    Main Results:

    • The modified Thomas electrode exhibits high sensitivity (57.9 mV/log aNa) and selectivity (KpotNa,K < 0.005).
    • The electrode has low electrical resistance (7.65 X 10(11) ohms) and a small sensing chamber (10 microns3).

    Conclusions:

    • The modified Thomas electrode is a viable tool for direct intracellular sodium concentration measurement in epithelial cells.
    • The electrode's characteristics make it suitable for detailed cellular sodium studies.