Plant Sample Collection and Shipment for Multi-omic Analyses and Phytosanitary Evaluation

Area of Science:

• Plant Science
• Genomics
• Metabolomics

Background:

• Accurate metabolomics requires quenching hydrolytic enzymes in plant samples.
• Standard DNA preservation methods are often compatible with metabolite preservation.

Purpose of the Study:

• To describe comprehensive protocols for combined plant genomics and metabolomics.
• To enable multi-omics data collection from single samples for large-scale crop studies.
• To provide protocols adaptable for fresh-frozen and freeze-dried plant materials, including long-distance transport.

Main Methods:

• Detailed procedures for sample collection, phytosanitary shipment, and processing.
• Protocols for preparing both freeze-dried and fresh-frozen plant materials for metabolomics.
• Methods for DNA extraction and quality control for various genotyping applications.
• Specific protocols for metabolite extraction and sample shipment for metabolomic analyses.

Main Results:

• Established protocols for simultaneous DNA and metabolite analysis from single plant samples.
• Validated methods for preserving sample integrity during long-distance transport.
• Adaptable protocols for both freeze-dried and fresh-frozen plant tissues.
• Successful generation of multi-omics datasets in large experimental setups.

Conclusions:

• Unified sample preparation strategies facilitate integrated genomics and metabolomics studies.
• The described protocols support robust multi-omics data acquisition from crop plants.
• These methods are crucial for accurate analysis of both genetic and metabolic profiles in plants.