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Preparation of a High-quality Primary Cell Culture from Fish Pituitaries
Published on: August 28, 2018
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Profiling the Physiological Roles in Fish Primary Cell Culture.
Lingjie He1, Cheng Zhao2, Qi Xiao1
1College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Biology
|December 22, 2023
Summary
Fish primary cell culture enables studying fish cell physiology in vitro. This review details advancements in culturing diverse fish cells for research on health, disease, and sustainable aquaculture.
Area of Science:
- Aquatic biology and cell science.
- In vitro models for fish physiology.
Background:
- Fish primary cell culture is crucial for understanding fish physiology and responses.
- Advancements have improved culture conditions, protocols, and characterization.
Purpose of the Study:
- To review fish primary cell culture advancements and applications.
- To highlight the profiling of physiological roles of fish cells in vitro.
- To explore applications in research and aquaculture.
Main Methods:
- Isolation and cultivation of cells directly from fish tissues.
- Optimization of culture conditions and standardization of protocols.
- Characterization of diverse fish cell types in vitro.
Main Results:
- Successful culturing of various fish cell types (gonad, pituitary, muscle, liver, kidney, immune, etc.).
- Demonstrated distinct physiological functions of cultured cells (metabolism, regeneration, immunity).
- Established utility in studying environmental factors, toxins, pathogens, and drugs.
Conclusions:
- Fish primary cell cultures are vital for elucidating physiological mechanisms.
- Applications extend to aquaculture research, aiding sustainable practices.
- In vitro models provide insights into fish health, disease, and performance.
