Five draft genome assemblies from Bacillaceae isolated from a degraded wetland environment
Anna L McLoon1, Prince Ackaah Asante1, Thomas Anderson1
1Department of Biology, Siena College, Loudonville, New York, USA.
Microbiology Resource Announcements
|December 22, 2023
Summary
Researchers sequenced the genomes of five Bacillaceae bacteria, including Bacillus velezensus and Priestia megaterium, isolated from a degraded wetland. This provides valuable genomic data for understanding microbial life in challenging environments.
Area of Science:
- Microbiology
- Genomics
- Environmental Science
Background:
- Degraded wetlands harbor diverse microbial communities.
- Bacillaceae are ubiquitous and play significant roles in various ecosystems.
- Genomic data is crucial for understanding microbial adaptation and function.
Purpose of the Study:
- To isolate and identify Bacillaceae from a degraded wetland.
- To perform whole-genome sequencing of selected isolates.
- To provide draft genome sequences for future research.
Main Methods:
- Isolation of bacteria from wetland soil samples.
- Genomic DNA extraction and purification.
- Whole-genome sequencing using Illumina NextSeq platform.
Main Results:
- Five Bacillaceae strains were successfully isolated and identified: Bacillus velezensus-SC119, Priestia megaterium SC120, Bacillus zhangzhouensis SC123, Bacillus pumilis SC124, and Bacillus idriensis SC127.
- Draft genome sequences were generated, with sizes ranging from 3,657,353 to 5,772,725 base pairs.
- GC content varied between 37.62% and 46.38% across the sequenced genomes.
Conclusions:
- The study presents the first draft genome sequences for these specific Bacillaceae strains from a degraded wetland.
- These genomic resources will facilitate further investigations into their ecological roles and potential applications.
- The findings contribute to the understanding of microbial diversity in stressed environments.
