Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...
Amperometry: Overview01:10

Amperometry: Overview

Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Miniaturizing Sensor Active Areas to Enhance Analyte Surface Densities by Increasing "Effective" Analyte Concentrations.

ACS omega·2026
Same author

A Graphene Field-Effect Transistor-Based Biosensor Platform for the Electrochemical Profiling of Amino Acids.

Biosensors·2026
Same author

Peptide-Based Plasmon-Enhanced Spectroscopic Immunoassay to Detect Immunity Against Cytomegalovirus.

Biosensors·2025
Same author

Antimicrobial Peptides Act-6 and Act 8-20 Derived from Scarabaeidae Cecropins Exhibit Differential Antifungal Activity.

Journal of fungi (Basel, Switzerland)·2025
Same author

Mining the heparinome for cryptic antimicrobial peptides that selectively kill Gram-negative bacteria.

Molecular systems biology·2025
Same author

Design of Small Non-Peptidic Ligands That Alter Heteromerization between Cannabinoid CB<sub>1</sub> and Serotonin 5HT<sub>2A</sub> Receptors.

Journal of medicinal chemistry·2024
Same journal

Correction: Jiang et al. Methods for Obtaining One Single Larmor Frequency, Either <i>v</i><sub>1</sub> or <i>v</i><sub>2</sub>, in the Coherent Spin Dynamics of Colloidal Quantum Dots. <i>Nanomaterials</i> 2023, <i>13</i>, 2006.

Nanomaterials (Basel, Switzerland)·2026
Same journal

Correction: Ekman et al. Synthesis, Characterization, and Adsorption Properties of Nitrogen-Doped Nanoporous Biochar: Efficient Removal of Reactive Orange 16 Dye and Colorful Effluents. <i>Nanomaterials</i> 2023, <i>13</i>, 2045.

Nanomaterials (Basel, Switzerland)·2026
Same journal

Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub>-Based Materials and Coatings for De-Icing and Defogging of Wind Turbine Blades: Materials Basis, Structural Design, Engineering Integration, and Future Opportunities.

Nanomaterials (Basel, Switzerland)·2026
Same journal

Influence of the Ripeness Stages of the Precursors on the Optical Characteristics of Carbon Dots Obtained from Valencia Orange Peels (<i>Citrus sinensis</i> L. Osbeck) by Hydrothermal Synthesis.

Nanomaterials (Basel, Switzerland)·2026
Same journal

Insights into ALD Growth of Al-Based Dielectric Stack on 4H-SiC.

Nanomaterials (Basel, Switzerland)·2026
Same journal

Metal-<i>N</i>-Heterocyclic Carbene Porous Organic Polymers as Efficient Bifunctional Water-Splitting Electrocatalysts.

Nanomaterials (Basel, Switzerland)·2026
See all related articles

Related Experiment Video

Updated: Jul 2, 2026

Bridging the Bio-Electronic Interface with Biofabrication
16:38

Bridging the Bio-Electronic Interface with Biofabrication

Published on: June 6, 2012

16.8K

Acid-Modulated Peptide Synthesis for Application on Oxide Biosensor Interfaces.

Edgar Cristóbal-Lecina1, Janwa El-Maiss2, Eduard Figueras1

  • 1Proteomics and Protein Chemistry Unit, Department of Medicine and Life Sciences, Pompeu Fabra University, 08003 Barcelona, Spain.

Nanomaterials (Basel, Switzerland)
|December 22, 2023
PubMed
Summary
This summary is machine-generated.

We developed an acid-modulated strategy for producing novel peptide microarrays on biosensor interfaces. This method enables label-free protein screening using field-effect transistor (FET) sensors with high peptide yield and purity.

Keywords:
SPR chipsacid-labile protection schemeglass surfacespeptide synthesis

More Related Videos

Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy
05:44

Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy

Published on: March 6, 2017

8.1K
A Tripeptide-Stabilized Nanoemulsion of Oleic Acid
10:42

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid

Published on: February 27, 2019

9.4K

Related Experiment Videos

Last Updated: Jul 2, 2026

Bridging the Bio-Electronic Interface with Biofabrication
16:38

Bridging the Bio-Electronic Interface with Biofabrication

Published on: June 6, 2012

16.8K
Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy
05:44

Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy

Published on: March 6, 2017

8.1K
A Tripeptide-Stabilized Nanoemulsion of Oleic Acid
10:42

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid

Published on: February 27, 2019

9.4K

Area of Science:

  • Biotechnology
  • Chemical Synthesis
  • Biosensor Technology

Background:

  • Solid-phase peptide synthesis (SPPS) is crucial for creating peptide-based tools.
  • Developing efficient synthesis methods for biosensor interfaces remains a challenge.
  • Label-free detection methods are highly desirable for protein screening.

Purpose of the Study:

  • To report a novel acid-modulated strategy for peptide microarray production.
  • To adapt SPPS chemistry for direct use on biosensor interfaces, specifically field-effect transistor (FET) sensors.
  • To enable label-free protein screening through the creation of peptide microarrays.

Main Methods:

  • Utilized controlled pore glass (CPG) as a support for SPPS.
  • Employed an acid-labile tert-butyloxycarbonyl (Boc) protecting group for N-terminal amino functions.
  • Combined Boc protection with semi-permanent side-chain protection for amino acid residues.
  • Adapted and optimized the protection scheme for CPG and translated it to surface plasmon resonance (SPR) chips.

Main Results:

  • Achieved high yields and purity of peptides synthesized on CPG supports.
  • Successfully demonstrated the feasibility of acid-modulated synthesis on flat glass surfaces.
  • Validated the layer-by-layer monitoring of amino acid coupling using SPR chips.
  • Established a viable method for producing peptide microarrays on bio-FET sensor interfaces.

Conclusions:

  • The acid-modulated strategy is effective for producing high-quality peptides on biosensor interfaces.
  • This approach facilitates the development of label-free peptide microarrays for protein screening applications.
  • The method is transferable to various flat glass surfaces, including those used in bio-FETs.